Long non‑coding RNA snaR is involved in the metastasis of liver cancer possibly through TGF‑β1

Shi,Zhitian; Wei,Dong; Wu,Huamei; Ge,Jiayun; Lei,Xuefen; Guo,Zhitang; Zou,Renchao; Xiao,Shufeng; Wu,Tiangen; Ma,Ruicheng; Ai,Runyao; Wang,Lin

doi:10.3892/ol.2019.10258

Long non‑coding RNA snaR is involved in the metastasis of liver cancer possibly through TGF‑β1

Authors:
- Zhitian Shi
- Dong Wei
- Huamei Wu
- Jiayun Ge
- Xuefen Lei
- Zhitang Guo
- Renchao Zou
- Shufeng Xiao
- Tiangen Wu
- Ruicheng Ma
- Runyao Ai
- Lin Wang
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Affiliations: Department of Hepatopancreatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, P.R. China, Department of Digestive System, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, P.R. China, Department of Oncology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, P.R. China
Published online on: April 16, 2019 https://doi.org/10.3892/ol.2019.10258
Pages: 5565-5571
Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

It was previously demonstrated that the long non‑coding RNA (lncRNA) small NF90‑associated RNA (snaR) served an oncogenic role in human colon cancer, although its roles in other types of cancer remain unknown. To investigate the potential involvement of lncRNA snaR in hepatocellular carcinoma (HCC), expression of snaR in liver biopsies and plasma of patients with HCC and healthy controls was detected by reverse transcription‑quantitative polymerase chain reaction. ELISA was used to determine the protein expression levels of transforming growth factor‑β1 (TGF‑β1). A snaR expression vector was transfected into HCC cells, and the effects on cell migration and invasion were analyzed by Transwell migration and Matrigel invasion assays, respectively. The protein expression levels of TGF‑β1 in HCC cells were detected by western blotting. The expression of snaR and TGF‑β1 was significantly increased in the patients with HCC compared with the healthy controls. The plasma expression levels of snaR and TGF‑β1 were positively correlated in patients with HCC; however, not in healthy controls. snaR overexpression significantly promoted cancer cell migration and invasion, and additionally increased TGF‑β1 expression. Treatment with TGF‑β1 did not significantly affect snaR expression. A TGF‑β1 inhibitor attenuated the effects of snaR overexpression in cancer cell migration and invasion. snaR may promote the metastasis of liver cancer through TGF‑β1.

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