miR‑330‑3p suppresses liver cancer cell migration by targeting MAP2K1

Jin,Zhe; Jia,Baoxing; Tan,Ludong; Liu,Yahui

doi:10.3892/ol.2019.10280

miR‑330‑3p suppresses liver cancer cell migration by targeting MAP2K1

Authors:
- Zhe Jin
- Baoxing Jia
- Ludong Tan
- Yahui Liu
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Affiliations: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: April 24, 2019 https://doi.org/10.3892/ol.2019.10280
Pages: 314-320

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Abstract

MicroRNAs, considered as a promising focus for the treatment of tumors, are key regulators of a large number of genes. The aim of the present study was to investigate the biological functions of microRNA (miR)‑330‑3p in liver cancer as it had been identified previously that miR‑330‑3p was deregulated in liver cancer. In order to identify the function of miR‑330‑3p in liver cancer, the expression of miR‑330‑3p was determined in liver cancer tissues and adjacent non‑tumor tissues using reverse transcription‑quantitative polymerase chain reaction analysis. To elucidate the function of miR‑330‑3p in liver cancer, miR‑330‑3p was overexpressed using mimic transfection. Cell migration was inhibited by miR‑330‑3p in liver cancer cells. The miRNA target prediction databases were used to identify potential target genes of miR‑330‑3p in liver cancer. The RNA level of mitogen‑activated protein kinase kinase 1 (MAP2K1) was downregulated by miR‑330‑3p in liver cancer cells. In conclusion, miR‑330‑3p suppresses cell migration by targeting MAP2K1 in liver cancer cells.

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1	Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar : PubMed/NCBI
2	Forner A, Hessheimer AJ, Isabel Real M and Bruix J: Treatment of hepatocellular carcinoma. Crit Rev Oncol Hematol. 60:89–98. 2006. View Article : Google Scholar : PubMed/NCBI
3	Jeng KS and Ching HJ: The role of surgery in the management of unusual complications of transcatheter arterial embolization for hepatocellular carcinoma. World J Surg. 12:362–368. 1988. View Article : Google Scholar : PubMed/NCBI
4	Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI
5	Ryan BM, Robles AI and Harris CC: Genetic variation in microRNA networks: The implications for cancer research. Nat Rev Cancer. 10:389–402. 2010. View Article : Google Scholar : PubMed/NCBI
6	Ma D, Tao X, Gao F, Fan C and Wu D: miR-224 functions as an onco-miRNA in hepatocellular carcinoma cells by activating AKT signaling. Oncol Lett. 4:483–488. 2012. View Article : Google Scholar : PubMed/NCBI
7	Liu H, Li W, Chen C, Pei Y and Long X: MiR-335 acts as a potential tumor suppressor miRNA via downregulating ROCK1 expression in hepatocellular carcinoma. Tumour Biol. 36:6313–6319. 2015. View Article : Google Scholar : PubMed/NCBI
8	Zhang Y, Li T, Guo P, Kang J, Wei Q, Jia X, Zhao W, Huai W, Qiu Y, Sun L and Han L: MiR-424-5p reversed epithelial-mesenchymal transition of anchorage-independent HCC cells by directly targeting ICAT and suppressed HCC progression. Sci Rep. 4:62482014. View Article : Google Scholar : PubMed/NCBI
9	Chen J, Wu X, Cai B, Su Z, Li L, An Y and Wang L: Circulating microRNAs as potential biomarkers of HBV infection persistence. Infect Genet Evol. 54:152–157. 2017. View Article : Google Scholar : PubMed/NCBI
10	Kenneth J and Livak TD: Analysis of relative gene expression data using rea l—time quantitative PCR a nd the 2 □ ct method. Method. 2001.
11	Vitali GC, Laurent A, Terraz S, Majno P, Buchs NC, Rubbia-Brandt L, Luciani A, Calderaro J, Morel P, Azoulay D and Toso C: Minimally invasive surgery versus percutaneous radio frequency ablation for the treatment of single small (</=3 cm) hepatocellular carcinoma: A case-control study. Surg Endosc. 30:2301–2307. 2016. View Article : Google Scholar : PubMed/NCBI
12	Xiong W, Sun LP, Chen XM, Li HY, Huang SA and Jie SH: Comparison of microRNA expression profiles in HCC-derived microvesicles and the parental cells and evaluation of their roles in HCC. J Huazhong Univ Sci Technolog Med Sci. 33:346–352. 2013. View Article : Google Scholar : PubMed/NCBI
13	Deng S, Calin GA, Croce CM, Coukos G and Zhang L: Mechanisms of microRNA deregulation in human cancer. Cell Cycle. 7:2643–2646. 2008. View Article : Google Scholar : PubMed/NCBI
14	Dinkova-Kostova AT, Jenkins SN, Fahey JW, Ye L, Wehage SL, Liby KT, Stephenson KK, Wade KL and Talalay P: Protection against UV-light-induced skin carcinogenesis in SKH-1 high-risk mice by sulforaphane-containing broccoli sprout extracts. Cancer Lett. 240:243–252. 2006. View Article : Google Scholar : PubMed/NCBI
15	Wei CH, Wu G, Cai Q, et al: MicroRNA-330-3p promotes cell invasion and metastasis in non-small cell lung cancer through GRIA3 by activating MAPK/ERK signaling pathway. J Hematol Oncol. 10:1252017. View Article : Google Scholar : PubMed/NCBI
16	Mao Y, Chen H, Lin Y, Xu X, Hu Z, Zhu Y, Wu J, Xu X, Zheng X and Xie L: microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells. Oncol Rep. 30:327–333. 2013. View Article : Google Scholar : PubMed/NCBI
17	Huynh H, Nguyen TT, Chow KH, Tan PH, Soo KC and Tran E: Over-expression of the mitogen-activated protein kinase (MAPK) kinase (MEK)-MAPK in hepatocellular carcinoma: Its role in tumor progression and apoptosis. BMC Gastroenterol. 3:192003. View Article : Google Scholar : PubMed/NCBI
18	Liang HH, Wei PL, Hung CS, Wu CT, Wang W, Huang MT and Chang YJ: MicroRNA-200a/b influenced the therapeutic effects of curcumin in hepatocellular carcinoma (HCC) cells. Tumour Biol. 34:3209–3218. 2013. View Article : Google Scholar : PubMed/NCBI
19	Chen X, Zhang L, Zhang T, Hao M, Zhang X, Zhang J, Xie Q, Wang Y, Guo M, Zhuang H and Lu F: Methylation-mediated repression of microRNA 129-2 enhances oncogenic SOX4 expression in HCC. Liver Int. 33:476–486. 2013. View Article : Google Scholar : PubMed/NCBI
20	Zhang D, Zhou P, Wang W, Wang X, Li J, Sun X and Zhang L: MicroRNA-616 promotes the migration, invasion and epithelial-mesenchymal transition of HCC by targeting PTEN. Oncol Rep. 35:366–374. 2016. View Article : Google Scholar : PubMed/NCBI
21	Paiva I, Gil da Costa RM, Ribeiro J, Sousa H, Bastos MM, Faustino-Rocha A, Lopes C, Oliveira PA and Medeiros R: MicroRNA-21 expression and susceptibility to HPV-induced carcinogenesis-role of microenvironment in K14-HPV16 mice model. Life Sci. 128:8–14. 2015. View Article : Google Scholar : PubMed/NCBI
22	Paiva I, Gil da Costa RM, Ribeiro J, Sousa H, Bastos M, Faustino-Rocha A, Lopes C, Oliveira PA and Medeiros R: A role for microRNA-155 expression in microenvironment associated to HPV-induced carcinogenesis in K14-HPV16 transgenic mice. PLoS One. 10:e01168682015. View Article : Google Scholar : PubMed/NCBI