Effect of docetaxel‑loaded lipid microbubble in combination with ultrasound‑triggered microbubble destruction on the growth of a gastric cancer cell line

  • Authors:
    • Bin Lai
    • Peiqian Zhu
    • Honglang Li
    • Lin Hu
    • Jiwei Wang
  • View Affiliations

  • Published online on: April 30, 2019     https://doi.org/10.3892/ol.2019.10289
  • Pages: 442-448
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Abstract

Although gastric cancer therapy has been improved, more efficient treatment strategies still need to be developed. In the present study, a docetaxel (DOC)‑loaded lipid microbubble (DLLD) was prepared and the effect of DLLD combined with ultrasound‑triggered microbubble destruction (UTMD) on the growth of a gastric cancer cell line was investigated. The following four groups were included in the present study: Control, DOC, DLLD and DLLD plus UTMD. The determined entrapment efficiency of DLLD is 76±3.5%. The present study demonstrated that treatment with DLLD plus UTMD could significantly inhibit the growth of the cultured gastric cancer cell line BGC‑823 via arresting the cell cycle in G2/M phase, inhibiting cell DNA synthesis, promoting cell apoptosis and disrupting mitochondrial membrane potential, as compared with treatment with DOC or DLLD alone. Furthermore, the expression of p53, p21 and Bax were identified to be significantly upregulated, while that of Bcl‑2 was significantly downregulated in the DLLD plus UTMD group. Therefore, treatment with DLLD plus UTMD was more efficient in inhibiting cell proliferation and inducing cell apoptosis in the gastric cancer cell line, when compared with treatment with DOC or DLLD alone, suggesting that DLLD plus UTMD could serve as a promising strategy for the treatment of gastric cancer.
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July-2019
Volume 18 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Lai B, Zhu P, Li H, Hu L and Wang J: Effect of docetaxel‑loaded lipid microbubble in combination with ultrasound‑triggered microbubble destruction on the growth of a gastric cancer cell line. Oncol Lett 18: 442-448, 2019
APA
Lai, B., Zhu, P., Li, H., Hu, L., & Wang, J. (2019). Effect of docetaxel‑loaded lipid microbubble in combination with ultrasound‑triggered microbubble destruction on the growth of a gastric cancer cell line. Oncology Letters, 18, 442-448. https://doi.org/10.3892/ol.2019.10289
MLA
Lai, B., Zhu, P., Li, H., Hu, L., Wang, J."Effect of docetaxel‑loaded lipid microbubble in combination with ultrasound‑triggered microbubble destruction on the growth of a gastric cancer cell line". Oncology Letters 18.1 (2019): 442-448.
Chicago
Lai, B., Zhu, P., Li, H., Hu, L., Wang, J."Effect of docetaxel‑loaded lipid microbubble in combination with ultrasound‑triggered microbubble destruction on the growth of a gastric cancer cell line". Oncology Letters 18, no. 1 (2019): 442-448. https://doi.org/10.3892/ol.2019.10289