Systematic re‑analysis strategy of serum indices identifies alkaline phosphatase as a potential predictive factor for cervical cancer

Yu,Jun; Zheng,Qianwen; Ding,Xiaoxing; Zheng,Bo; Chen,Xia; Chen,Binghai; Shen,Cong; Zhang,Yu; Luan,Xiaojin; Yan,Yidan; Chen,Wanyin; Xie,Bing; Wang,Min; Liu,Jiajia; Fang,Jie; Hu,Xing; Li,Hong; Qiao,Chen; Yang,Peifang

doi:10.3892/ol.2019.10527

Systematic re‑analysis strategy of serum indices identifies alkaline phosphatase as a potential predictive factor for cervical cancer

Authors:
- Jun Yu
- Qianwen Zheng
- Xiaoxing Ding
- Bo Zheng
- Xia Chen
- Binghai Chen
- Cong Shen
- Yu Zhang
- Xiaojin Luan
- Yidan Yan
- Wanyin Chen
- Bing Xie
- Min Wang
- Jiajia Liu
- Jie Fang
- Xing Hu
- Hong Li
- Chen Qiao
- Peifang Yang
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Affiliations: Department of Gynecology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China, Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, P.R. China, Department of Urology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China, Department of Clinical Pharmacy, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China
Published online on: June 26, 2019 https://doi.org/10.3892/ol.2019.10527
Pages: 2356-2365
Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to identify predictive factors for cervical cancer (CC) progression using a multistage approach. The present study obtained data from 390 healthy women and 259 patients with cervical cancer between June 2012 and June 2017, and used a multiple stage re‑analysis strategy for clinical detection of CC. A total of seven types of serum indices were used in the present study, including sugar chain antigen 125 (CA‑125), sugar chain antigen 199 (CA‑199), α fetoprotein (AFP), carcino‑embryonic antigen, alkaline phosphatase (ALP), cholesterol and triglyceride (TG). The expression levels of CA‑125, CA‑199, AFP, ALP, cholesterol and TG were significantly different between healthy women and patients with cervical squamous cell carcinoma (SCC). Furthermore, ALP, cholesterol and TG expression levels were significantly different in healthy women compared with patients with cervical adenocarcinoma (AC). Further comparisons based on age and pathological staging demonstrated that the variability in the ALP level was not significant between the <40 years old age group and the 40‑50 years old age group within healthy individuals (P>0.05); however, was significant in patients with SCC (P<0.05). Staging analysis identified significant differences in ALP between healthy women and patients with SCC (Stage I‑IV), and significant differences between healthy women and patients with Stage I AC. The results of the present study indicated that the expression of ALP was significantly increased in patients with CC compared with healthy women. Therefore, ALP may be a potential predictive factor for the development of CC.

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1	Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI
2	Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI
3	Chen P, Jiao L and Wang DB: Squamous cell carcinoma antigen expression in tumor cells is associated with the chemosensitivity and survival of patients with cervical cancer receiving docetaxel-carboplatin-based neoadjuvant chemotherapy. Oncol Lett. 13:1235–1241. 2017. View Article : Google Scholar : PubMed/NCBI
4	Molina R, Filella X, Augé JM, Fuentes R, Bover I, Rifa J, Moreno V, Canals E, Viñolas N, Marquez A, et al: Tumor markers (CEA, CA 125, CYFRA 21-1, SCC and NSE) in patients with non-small cell lung cancer as an aid in histological diagnosis and prognosis. Comparison with the main clinical and pathological prognostic factors. Tumour Biol. 24:209–218. 2003. View Article : Google Scholar : PubMed/NCBI
5	Catanzaro JM, Guerriero JL, Liu J, Ullman E, Sheshadri N, Chen JJ and Zong WX: Elevated expression of squamous cell carcinoma antigen (SCCA) is associated with human breast carcinoma. PLoS One. 6:e190962011. View Article : Google Scholar : PubMed/NCBI
6	Sánchez Vega JF, Murillo Bacilio MDR, Vintimilla Condoy AS, Palta González AM, Crespo Astudillo JA and Mora-Bravo FG: Predictive equation of metastasis in patients with malignant ovarian epithelial tumors with the Ca-125 marker. BMC Cancer. 18:5872018. View Article : Google Scholar : PubMed/NCBI
7	Malati T: Tumour markers: An overview. Indian J Clin Biochem. 22:17–31. 2007. View Article : Google Scholar : PubMed/NCBI
8	Lebrecht A, Ludwig E, Huber A, Klein M, Schneeberger C, Tempfer C, Koelbl H and Hefler L: Serum vascular endothelial growth factor and serum leptin in patients with cervical cancer. Gynecol Oncol. 85:32–35. 2002. View Article : Google Scholar : PubMed/NCBI
9	Fuso L, Mazzola S, Marocco F, Ferrero A, Dompè D, Carus AP and Zola P: Pretreatment serum hemoglobin level as a predictive factor of response to neoadjuvant chemotherapy in patients with locally advanced squamous cervical carcinoma: A preliminary report. Gynecol Oncol. 99 (Suppl 1):S187–S191. 2005. View Article : Google Scholar : PubMed/NCBI
10	Bhatla N, Aoki D, Sharma DN and Sankaranarayanan R: Cancer of the cervix uteri. Int J Gynaecol Obstet. 143 (Suppl 2):S22–S36. 2018. View Article : Google Scholar
11	US Preventive Services Task Force, ; Curry SJ, Krist AH, Owens DK, Barry MJ, Caughey AB, Davidson KW, Doubeni CA, Epling JW Jr, Kemper AR, et al: Screening for cervical cancer: US preventive services task force recommendation statement. JAMA. 320:674–686. 2018. View Article : Google Scholar : PubMed/NCBI
12	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
13	Herfs M, Yamamoto Y, Laury A, Wang X, Nucci MR, McLaughlin-Drubin ME, Münger K, Feldman S, McKeon FD, Xian W and Crum CP: A discrete population of squamocolumnar junction cells implicated in the pathogenesis of cervical cancer. Proc Natl Acad Sci USA. 109:10516–10521. 2012. View Article : Google Scholar : PubMed/NCBI
14	Quinn BA, Deng X, Colton A, Bandyopadhyay D, Carter JS and Fields EC: Increasing age predicts poor cervical cancer prognosis with subsequent effect on treatment and overall survival. Brachytherapy. 18:29–37. 2019. View Article : Google Scholar : PubMed/NCBI
15	Zhang C, Wang H, Ning Z, Xu L, Zhuang L, Wang P and Meng Z: Serum liver enzymes serve as prognostic factors in patients with intrahepatic cholangiocarcinoma. Onco Targets Ther. 10:1441–1449. 2017. View Article : Google Scholar : PubMed/NCBI
16	Fuchs CS, Muro K, Tomasek J, Van Cutsem E, Cho JY, Oh SC, Safran H, Bodoky G, Chau I, Shimada Y, et al: Prognostic factor analysis of overall survival in gastric cancer from two phase III studies of second-line ramucirumab (REGARD and RAINBOW) using pooled patient data. J Gastric Cancer. 17:132–144. 2017. View Article : Google Scholar : PubMed/NCBI
17	Haarhaus M, Brandenburg V, Kalantar-Zadeh K, Stenvinkel P and Magnusson P: Alkaline phosphatase: A novel treatment target for cardiovascular disease in CKD. Nat Rev Nephrol. 13:429–442. 2017. View Article : Google Scholar : PubMed/NCBI
18	Magnusson P, Degerblad M, Sääf M, Larsson L and Thorén M: Different responses of bone alkaline phosphatase isoforms during recombinant insulin-like growth factor-i (IGF-I) and during growth hormone therapy in adults with growth hormone deficiency. J Bone Miner Res. 12:210–220. 1997. View Article : Google Scholar : PubMed/NCBI
19	Metwalli AR, Rosner IL, Cullen J, Chen Y, Brand T, Brassell SA, Lesperance J, Porter C, Sterbis J and McLeod DG: Elevated alkaline phosphatase velocity strongly predicts overall survival and the risk of bone metastases in castrate-resistant prostate cancer. Urol Oncol. 32:761–768. 2014. View Article : Google Scholar : PubMed/NCBI
20	Sonpavde G, Pond GR, Berry WR, de Wit R, Armstrong AJ, Eisenberger MA and Tannock IF: Serum alkaline phosphatase changes predict survival independent of PSA changes in men with castration-resistant prostate cancer and bone metastasis receiving chemotherapy. Urol Oncol. 30:607–613. 2012. View Article : Google Scholar : PubMed/NCBI
21	Fedde KN, Lane CC and Whyte MP: Alkaline phosphatase is an ectoenzyme that acts on micromolar concentrations of natural substrates at physiologic pH in human osteosarcoma (SAOS-2) cells. Arch Biochem Biophys. 264:400–409. 1998. View Article : Google Scholar
22	Rao SR, Snaith AE, Marino D, Cheng X, Lwin ST, Orriss IR, Hamdy FC and Edwards CM: Tumour-derived alkaline phosphatase regulates tumour growth, epithelial plasticity and disease-free survival in metastatic prostate cancer. Br J Cancer. 116:227–236. 2017. View Article : Google Scholar : PubMed/NCBI
23	Millán JL: The role of phosphatases in the initiation of skeletal mineralization. Calcif Tissue Int. 93:299–306. 2013. View Article : Google Scholar : PubMed/NCBI
24	Nam HK, Sharma M, Liu J and Hatch NE: Tissue nonspecific alkaline phosphatase (TNAP) regulates cranial base growth and synchondrosis maturation. Front Physiol. 8:1612017. View Article : Google Scholar : PubMed/NCBI
25	Shao J, Wang C, Li L, Liang H, Dai J, Ling X and Tang H: Luteoloside inhibits proliferation and promotes intrinsic and extrinsic pathway-mediated apoptosis involving MAPK and mTOR signaling pathways in human cervical cancer cells. Int J Mol Sci. 19:E16642018. View Article : Google Scholar : PubMed/NCBI
26	Hua FF, Liu SS, Zhu LH, Wang YH, Liang X, Ma N and Shi HR: MiRNA-338-3p regulates cervical cancer cells proliferation by targeting MACC1 through MAPK signaling pathway. Eur Rev Med Pharmacol Sci. 21:5342–5352. 2017.PubMed/NCBI
27	Sun Q, Liang Y, Zhang T, Wang K and Yang X: ER-α36 mediates estrogen-stimulated MAPK/ERK activation and regulates migration, invasion, proliferation in cervical cancer cells. Biochem Biophys Res Commun. 487:625–632. 2017. View Article : Google Scholar : PubMed/NCBI
28	Li S, Ma YM, Zheng PS and Zhang P: GDF15 promotes the proliferation of cervical cancer cells by phosphorylating AKT1 and Erk1/2 through the receptor ErbB2. J Exp Clin Cancer Res. 37:802018. View Article : Google Scholar : PubMed/NCBI
29	Yang XL, Liu KY, Lin FJ, Shi HM and Ou ZL: CCL28 promotes breast cancer growth and metastasis through MAPK-mediated cellular anti-apoptosis and pro-metastasis. Oncol Rep. 38:1393–1401. 2017. View Article : Google Scholar : PubMed/NCBI
30	Wang C, Li P, Xuan J, Zhu C, Liu J, Shan L, Du Q, Ren Y and Ye J: Cholesterol enhances colorectal cancer progression via ROS elevation and MAPK signaling pathway activation. Cell Physiol Biochem. 42:729–742. 2017. View Article : Google Scholar : PubMed/NCBI
31	Zhang Z, Wang L, Du J, Li Y, Yang H, Li C, Li H and Hu H: Lipid raft localization of epidermal growth factor receptor alters matrix metalloproteinase-1 expression in SiHa cells via the MAPK/ERK signaling pathway. Oncol Lett. 12:4991–4998. 2016. View Article : Google Scholar : PubMed/NCBI
32	Cohen PA, Jhingran A, Oaknin A and Denny L: Cervical cancer. Lancet. 393:169–182. 2019. View Article : Google Scholar : PubMed/NCBI