miR‑148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer

Zhao,Wei; Zheng,Jianbao; Wei,Guangbing; Yang,Kui; Wang,Guanghui; Sun,Xuejun

doi:10.3892/ol.2019.10581

miR‑148a inhibits cell proliferation and migration through targeting ErbB3 in colorectal cancer

Authors:
- Wei Zhao
- Jianbao Zheng
- Guangbing Wei
- Kui Yang
- Guanghui Wang
- Xuejun Sun
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Affiliations: Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
Published online on: July 5, 2019 https://doi.org/10.3892/ol.2019.10581
Pages: 2530-2536
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colorectal cancer is a common gastrointestinal cancer ranking in third place of all cancers. Downregulation of miR‑148a has been observed in many tumors, and miR‑148a was found to be an oncogene in colorectal cancer. The aim of our study was to investigate the molecular mechanisms by which miR‑148a and ErbB3 proliferate and migrate in colorectal cancer. The expression of miR‑148a and ErbB3 were measured by western blot analysis and RT‑qPCR. MTT and transwell assays were performed to analyze the proliferative and migratory abilities. The dual luciferase reporter assay was employed to confirm miR‑148a regulated the expression of ErbB3 in colorectal cancer. It was discovered that miR‑148a was overexpressed while ErbB3 expression was low in colorectal cancer, and the mRNA level of miR‑148a had a negative correlation with the expression of ErbB3. Upregulation of miR‑148a suppressed the proliferation and migration in colorectal cancer cells. Furthermore, ErbB3 was identified as a direct target of miR‑148a, which suppressed the proliferation and migration through directly binding to the 3'UTR of ErbB3 mRNA. This study established that miR‑148a inhibited the proliferative and migratory abilities through mediating the expression of ErbB3. The newly identified miR‑148a/ErbB3 axis provides novel insight into the pathogenesis of colorectal cancer, and represents a potential target for treatment of colorectal cancer.

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