lncRNA LINC‑PINT is downregulated in melanoma and regulates cell proliferation by downregulating lncRNA BANCR

Huang,Qing; Zhang,Deli; Diao,Qingchun; Lin,Mao

doi:10.3892/ol.2019.10631

lncRNA LINC‑PINT is downregulated in melanoma and regulates cell proliferation by downregulating lncRNA BANCR

Authors:
- Qing Huang
- Deli Zhang
- Qingchun Diao
- Mao Lin
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Affiliations: Department of Dermatology, Chongqing Shapingba District People's Hospital, Chongqing 400011, P.R. China, Department of Dermatology, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400011, P.R. China
Published online on: July 18, 2019 https://doi.org/10.3892/ol.2019.10631
Pages: 2917-2922
Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The development of melanoma may involve long non‑coding RNAs (lncRNAs); however, the functions of the majority of lncRNAs in melanoma are unknown. The present study investigated the role of long intergenic non‑protein coding RNA p53 induced transcript (LINC‑PINT) in melanoma. In the present study, quantitative PCR was used to detect gene expression, overexpression experiments were performed to analyze gene interactions and CCK‑8 assays were used to analyze cell proliferation. LINC‑PINT was downregulated, while BRAF‑activated non‑coding RNA (BANCR) was upregulated in melanoma tissues compared with normal adjacent tissues. Expression levels of LINC‑PINT decreased, while expression levels of BANCR increased with increasing tumor thickness. The expression levels of LINC‑PINT and BANCR were inversely associated in melanoma tissues but not in healthy adjacent tissue. LINC‑PINT overexpression downregulated BANCR expression in melanoma cells, while BANCR overexpression did not significantly affect LINC‑PINT expression. LINC‑PINT overexpression inhibited melanoma cell proliferation in vitro compared to controls. BANCR overexpression attenuated the effects of LINC‑PINT overexpression. The present study revealed that lncRNA LINC‑PINT is downregulated in melanoma and may regulate melanoma cell proliferation by downregulating lncRNA BANCR.

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