Upregulation of microRNA‑520a‑3p inhibits the proliferation, migration and invasion via spindle and kinetochore associated 2 in gastric cancer

  • Authors:
    • Hui Su
    • Feng Ren
    • Haitao Jiang
    • Yunjie Chen
    • Xiaoxiang Fan
  • View Affiliations

  • Published online on: July 25, 2019     https://doi.org/10.3892/ol.2019.10663
  • Pages: 3323-3330
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Abstract

MicroRNAs (miR) serve important roles in the development and progression of tumors by targeting different genes. miR‑520a‑3p reported in lung and breast cancers as a tumor suppressor gene. However, the expression and functional significance of miR‑520a‑3p is not completely understood in gastric cancer (GC). In the present study, it was demonstrated that the expression levels of miR‑520a‑3p were significantly downregulated in GC tissues and cells using RT‑qPCR. In addition, downregulated expression of miR‑520a‑3p was associated with the clinical stage of the tumor and invasion in patients with GC. Furthermore, overexpression of miR‑520a‑3p significantly inhibited cell proliferation, invasion and migration in SGC‑7901 and MGC‑803 GC cell lines using proliferation, wound healing and cell invasion assays. Spindle and kinetochore associated 2 (SKA2) was upregulated in GC cells using western blot analysis and a target gene of miR‑520a‑3p; miR‑520a‑3p mimics significantly reduced SKA2 expression. In addition, upregulation of SKA2 protein expression SKA2 reversed the miR‑520a‑3p‑mediated inhibition of SGC‑7901 cell proliferation, migration and invasion. In conclusion, miR‑520a‑3p functioned as a tumor suppressor gene by targeting SKA2 in GC cell lines, and may serve as a novel prognostic and potential therapeutic marker.

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September 2019
Volume 18 Issue 3

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Copy and paste a formatted citation
APA
Su, H., Ren, F., Jiang, H., Chen, Y., & Fan, X. (2019). Upregulation of microRNA‑520a‑3p inhibits the proliferation, migration and invasion via spindle and kinetochore associated 2 in gastric cancer. Oncology Letters, 18, 3323-3330. https://doi.org/10.3892/ol.2019.10663
MLA
Su, H., Ren, F., Jiang, H., Chen, Y., Fan, X."Upregulation of microRNA‑520a‑3p inhibits the proliferation, migration and invasion via spindle and kinetochore associated 2 in gastric cancer". Oncology Letters 18.3 (2019): 3323-3330.
Chicago
Su, H., Ren, F., Jiang, H., Chen, Y., Fan, X."Upregulation of microRNA‑520a‑3p inhibits the proliferation, migration and invasion via spindle and kinetochore associated 2 in gastric cancer". Oncology Letters 18, no. 3 (2019): 3323-3330. https://doi.org/10.3892/ol.2019.10663