8-Hydroxy-2'-deoxyguanosine expression predicts hepatocellular carcinoma outcome

Li,Shengmian; Wang,Xiaoling; Wu,Yijuan; Zhang,Huijing; Zhang,Lan; Wang,Cuiju; Zhang,Ruixing; Guo,Zhanjun

doi:10.3892/ol.2011.477

8-Hydroxy-2'-deoxyguanosine expression predicts hepatocellular carcinoma outcome

Authors:
- Shengmian Li
- Xiaoling Wang
- Yijuan Wu
- Huijing Zhang
- Lan Zhang
- Cuiju Wang
- Ruixing Zhang
- Zhanjun Guo
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Affiliations: Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, P.R. China, Department of Pathology, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, P.R. China, Department of Gynecology Ultrasound, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, P.R. China, Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, P.R. China
Published online on: November 10, 2011 https://doi.org/10.3892/ol.2011.477
Pages: 338-342

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Abstract

Hepatocellular carcinoma (HCC) is characterized by increased oxidative stress and the production of 8-hydroxy-2'-deoxyguanosine (8-OHdG), which is one of the main mutagenic modifications of DNA by oxidative stress. We analyzed the association of 8-OHdG with post-operative survival and revealed that low levels of 8-OHdG are associated with significantly shorter survival time. Moreover, levels of 8-OHdG were associated with HCC characteristics, including tumor size, tumor quantity, clinical staging, Child classification, portal vein thrombosis and ascites. These results suggest that oxidative damage is a useful prognostic marker in HCC when other clinical characteristics are present with 8-OHdG.

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1	Ames BN, Shigenaga MK and Hagen TM: Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci USA. 90:7915–7922. 1993. View Article : Google Scholar : PubMed/NCBI
2	Shigenaga M, Gimeno CJ and Ames BN: Urinary 8-hydroxy-2′-deoxyguanosine as a biomarker of in vivo oxidative DNA damage. Proc Natl Acad Sci USA. 86:9697–9701. 1989.
3	Halliwell B: Why and how should we measure oxidative damage in nutritional studies? How far could you come? Am J Clin Nutr. 72:1082–1087. 2000.PubMed/NCBI
4	Nakae D, Kobayashi Y, Akai H, Andoh N, Satoh H, Ohashi K, Tsutsumi M and Konishi Y: Involvement of 8-hydroxyguanine formation in the initiation of rat liver carcinogenesis by low dose levels of N-nitrosodiethylamine. Cancer Res. 57:1281–1287. 1997.PubMed/NCBI
5	Kasai H: Analysis of a form of oxidative DNA damage 8-hydroxy-2′-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutat Res. 387:147–163. 1997.
6	Block TM, Mehta AS, Fimmel CJ and Jordan R: Molecular viral oncology of hepetocellular carcinoma. Oncogene. 22:5093–5107. 2003. View Article : Google Scholar : PubMed/NCBI
7	Waris G and Siddiqui A: Regulatory mechanisms of viral hepatitis B and C. J Biosci. 28:311–321. 2003. View Article : Google Scholar : PubMed/NCBI
8	Schwarz KB: Oxidative stress during viral infection: a review. Free Radical Biol Med. 21:641–649. 1996. View Article : Google Scholar : PubMed/NCBI
9	Tanaka H, Fujita N, Sugimoto R, Urawa N, Horiike S, Kobayashi Y, Iwasa M, Ma N, Kawanishi S, Watanabe S, Kaito M and Takei Y: Hepatic oxidative DNA damage is associated with increased risk for hepatocellular carcinoma in chronic hepatitis C. Br J Cancer. 98:580–586. 2008. View Article : Google Scholar : PubMed/NCBI
10	Hussain SP, Hofseth LJ and Harris CC: Radical causes of cancer. Nat Rev Cancer. 3:276–285. 2003. View Article : Google Scholar : PubMed/NCBI
11	De Maria N, Colantoni A, Fagiuoli S, Liu GJ, Rogers BK, Farinati F, Van Thiel DH and Floyd RA: Association between reactive oxygen species and disease activity in chronic hepatitis C. Free Radic Biol Med. 21:191–295. 1996.PubMed/NCBI
12	Chuma M, Hige S, Nakanishi M, Ogawa K, Natsuizaka M, Yamamoto Y and Asaka M: 8-hydroxy-2′-deoxyguanosine is a risk factor for development of hepatocellular carcinoma in patients with chronic hepatitis C virus infection. J Gastroenterol Hepatol. 23:1431–1436. 2008.
13	Maki A, Kono H, Gupta M, Asakawa M, Suzuki T, Matsuda M, Fujii H and Rusyn I: Predictive power of biomarkers of oxidative stress and inflammation in patients with hepatitis C virus-associated hepatocellular carcinoma. Anals Surg Oncol. 14:1182–1190. 2007. View Article : Google Scholar : PubMed/NCBI
14	Okada S, Shimada K, Yamamoto J, Takayama T, Kosuge T, Yamasaki S, Sakamoto M and Hirohashi S: Predictive factors for postoperative recurrence of hepatocellular carcinoma. Gastroenterology. 106:1618–1624. 1994.PubMed/NCBI
15	Minagawa M, Makuuchi M, Takayama T and Kokudo N: Selection criteria for repeat hepatectomy in patients with recurrent hepatocellular carcinoma. Ann Surg. 238:703–710. 2003. View Article : Google Scholar : PubMed/NCBI
16	Tung-Ping Poon R, Fan ST and Wong J: Risk factors, prevention, and management of postoperative recurrence after resection of hepatocellular carcinoma. Ann Surg. 232:10–24. 2000.PubMed/NCBI
17	Miyake H, Hara I, Kamidono S and Eto H: Prognostic significance of oxidative DNA damage evaluated by 8-hydroxy-2′-deoxyguanosine in patients undergoing radical nephrectomy for renal cell carcinoma. Urology. 64:1057–1061. 2004.PubMed/NCBI
18	Murtas D, Piras F, Minerba L, Uglade J, Floris C, Maxia C, Demurtas P, Perra MT and Sirigu P: Nuclear 8-hydroxy-2′-deoxyguanosine as survival biomarker in patients with cutaneous melanoma. Oncol Rep. 23:329–335. 2010.
19	Karihtala P, Soini Y, Vaskivuo L, Bloigu R and Puistola U: DNA adduct 8-Hydroxyguanosine, a novel putative marker of prognostic significance in ovarian carcinoma. Int J Gynecol Cancer. 19:1047–1051. 2009. View Article : Google Scholar : PubMed/NCBI
20	Floyd RA: The role of 8-hydroxyguanine in carcinogenesis. Carcinogenesis. 11:1447–1450. 1990. View Article : Google Scholar : PubMed/NCBI
21	Tanaka T, Kondo S, Iwasa Y, Hiai H and Toyokuni S: Expression of stress response and cell proliferation genes in renal cell carcinoma induced by oxidative stress. Am J Pathol. 156:2149–2157. 2000. View Article : Google Scholar : PubMed/NCBI
22	Inoue S and Kawanishi S: Oxidative DNA damage induced by simultaneous generation of nitric oxide and superoxide. FEBS Lett. 371:86–88. 1995. View Article : Google Scholar : PubMed/NCBI
23	Erhola M, Toyokuni S, Okada K, Tanaka H, Hiai H, Ochi K, Uchida T, Osawa MM, Nieminen H, Alho P and Kellokumpu-Lehtinen P: Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8-hydroxy-2′-deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment. FEBS Lett. 409:287–291. 1997.PubMed/NCBI
24	Miyake H, Hara I, Gleave ME and Eto H: Protection of androgen-dependent human prostate cancer cells from oxidative stress-induced DNA damage by overexpression of clusterinand its modulation by androgen. Prostate. 61:318–323. 2004. View Article : Google Scholar : PubMed/NCBI
25	Barash H, Gross E, Edrei Y, Israel A, Cohen I, Corchia N, Ben-Moshe T, Pappo O, Pikarsky E, Goldenberg D, Shiloh Y, Galun E and Abramovitch R: Accelerated carcinogenesis following liver regeneration is associated with chronic inflammation-induced double-strand DNA breaks. Proc Natl Acad Sci USA. 107:2207–2212. 2010. View Article : Google Scholar
26	Fujita N, Horiike S, Sugimoto R, Tanaka H, Iwasa M, Kobayashi Y, Hasegawa K, Ma N, Kawanishi S, Adachi Y and Kaito M: Hepatic oxidative DNA damage correlates with iron overload in chronic hepatitis C patients. Free Radic Biol Med. 42:353–362. 2007. View Article : Google Scholar : PubMed/NCBI
27	Poli G and Parola M: Oxidative damage and fibrogenesis. Free Radic Biol Med. 22:287–305. 1997. View Article : Google Scholar : PubMed/NCBI
28	Sumida Y, Nagashima M, Yoh T, Nakajima Y, Ishikawa H, Mitsuyoshi H, Sakamoto Y, Okanoue T, Kashima K, Nakamura H and Yodoi J: Serum thioredoxin levels as an indicator of oxidative stress in patients with hepatitis. J Hepatol. 33:616–622. 2000. View Article : Google Scholar : PubMed/NCBI
29	Collins A, Cadet J, Epe B and Gedik C: Problems in the measurement of 8-oxoguanine in human DNA. In: Report of a workshop, DNA oxidation; Aberdeen, UK. 19–21 January 1997; Carcinogenesis. 18. pp. 1833–1836. 1997, PubMed/NCBI