microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN

Mao,Jian  Hua; Zhou,Rong  Ping; Peng,Ai   Fen; Liu,Zhi  Li; Huang,Shan   Hu; Long,Xin  Hua; Shu,Yong

doi:10.3892/ol.2012.863

microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN

Authors:
- Jian Hua Mao
- Rong Ping Zhou
- Ai Fen Peng
- Zhi Li Liu
- Shan Hu Huang
- Xin Hua Long
- Yong Shu
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Affiliations: Department of Orthopedics, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Orthopedics, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, School of Humanities, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330006, P.R. China
Published online on: August 16, 2012 https://doi.org/10.3892/ol.2012.863
Pages: 1125-1129

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Abstract

microRNAs are involved in different cancer-related processes. miR-195, one of the miR-16/15/195/424/497 family members, has been shown to act as a tumor suppressor during tumorigenesis. However, the function of miR-195 in osteosarcoma is still unclear. In our study, the miR-195 expression level was upregulated in osteosarcoma cells, by transfection with miR-195, and the fatty acid synthase (FASN) mRNA and protein expression levels were measured by RT-PCR and western blotting. Cell migration and invasion was measured using wound healing migration and Transwell invasion assays. We found that the upregulation of miR-195 greatly decreased cell invasion and the migration of U2OS. We also identified that FASN may be a direct target of miR-195 by the luciferase activity assay. These findings provide evidence that miR-195 plays a key role in inhibiting osteosarcoma cell migration and invasion through targeting FASN, and strongly suggest that exogenous miR-195 may have therapeutic value in treating osteosarcoma.

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