ID1 regulates U87 human cell proliferation and invasion

  • Authors:
    • Pin Guo
    • Jin Lan
    • Jianwei Ge
    • Qing Mao
    • Yongming Qiu
  • View Affiliations

  • Published online on: August 1, 2013     https://doi.org/10.3892/ol.2013.1507
  • Pages: 921-926
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Despite therapeutic advances, the prognosis of patients diagnosed with malignant glioma has not improved in recent years. In particular, the molecular mechanisms that mediate glioma invasion remain poorly understood. The importance of ID1 in promoting tumor invasion and metastasis has recently emerged and a role for ID1 as a possible molecular marker of tumor aggressiveness has been proposed. To investigate the biological function of ID1 in glioblastomas, ID1‑silenced U87 glioblastoma multiforme (GBM) cells were constructed using a small hairpin RNA (shRNA) sequence. The effect of the knockdown of ID1 on proliferation and invasion in these cells was analyzed using the 5‑bromo‑2'‑deoxy‑uridine cell proliferation, Transwell invasion, scratch and cell adhesion assays. Compared with the controls, the U87 cells expressing ID1‑shRNA exhibited a significantly decreased proliferation and invasion capacity (P<0.05), as well as increased cell adhesion. Furthermore, silencing ID1 reduced the expression of c‑Myc, cyclin D1 and β‑catenin, while increasing E‑cadherin expression in U87 cells. This study showed that ID1 regulates the metastatic potential of GBM cells by controlling the epithelial‑mesenchymal transition. Therefore, ID1 is a potential prognostic indicator and therapeutic target in glioblastomas.
View Figures
View References

Related Articles

Journal Cover

October 2013
Volume 6 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Guo P, Lan J, Ge J, Mao Q and Qiu Y: ID1 regulates U87 human cell proliferation and invasion. Oncol Lett 6: 921-926, 2013.
APA
Guo, P., Lan, J., Ge, J., Mao, Q., & Qiu, Y. (2013). ID1 regulates U87 human cell proliferation and invasion. Oncology Letters, 6, 921-926. https://doi.org/10.3892/ol.2013.1507
MLA
Guo, P., Lan, J., Ge, J., Mao, Q., Qiu, Y."ID1 regulates U87 human cell proliferation and invasion". Oncology Letters 6.4 (2013): 921-926.
Chicago
Guo, P., Lan, J., Ge, J., Mao, Q., Qiu, Y."ID1 regulates U87 human cell proliferation and invasion". Oncology Letters 6, no. 4 (2013): 921-926. https://doi.org/10.3892/ol.2013.1507