Pro‑apoptotic and migration‑suppressing potential of EGCG, and the involvement of AMPK in the p53‑mediated modulation of VEGF and MMP‑9 expression

  • Authors:
    • Song Yi Park
    • Chang Hee Jung
    • Bokyung Song
    • Ock Jin Park
    • Young‑Min Kim
  • View Affiliations

  • Published online on: August 19, 2013     https://doi.org/10.3892/ol.2013.1533
  • Pages: 1346-1350
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Abstract

The present study investigated the regulatory mechanisms by which epigallocatechin‑3‑gallate (EGCG) exerts vascular endothelial growth factor (VEGF)‑, p53‑ and AMP‑activated protein kinase (AMPK)‑associated pro‑apoptotic and migration‑suppressing effects on colon cancer cells. EGCG decreased the expression levels of VEGF and matrix metalloproteinase (MMP)‑9. EGCG treatment induced apoptosis in the presence of wild‑type and mutant p53, indicating that a p53‑independent pathway may contribute to EGCG‑induced apoptosis in these cells. EGCG showed migration‑suppressing effects, suggesting that this activity may also have p53‑dependent and ‑independent components. The interaction between p53 and VEGF in the EGCG‑treated cells was investigated using pifithrin‑α. Notably, the suppression of p53 activity blocked the ability of EGCG to inhibit VEGF and MMP‑9 in the cells expressing wild‑type p53, but not mutant p53, indicating that the effects of EGCG on VEGF may be p53‑dependent or ‑independent. Finally, although AMPK and VEGF did not appear to co‑localize, the results indicated that AMPK controls VEGF in EGCG‑treated cells regardless of the p53 status.
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November 2013
Volume 6 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Park SY, Jung CH, Song B, Park OJ and Kim YM: Pro‑apoptotic and migration‑suppressing potential of EGCG, and the involvement of AMPK in the p53‑mediated modulation of VEGF and MMP‑9 expression. Oncol Lett 6: 1346-1350, 2013.
APA
Park, S.Y., Jung, C.H., Song, B., Park, O.J., & Kim, Y. (2013). Pro‑apoptotic and migration‑suppressing potential of EGCG, and the involvement of AMPK in the p53‑mediated modulation of VEGF and MMP‑9 expression. Oncology Letters, 6, 1346-1350. https://doi.org/10.3892/ol.2013.1533
MLA
Park, S. Y., Jung, C. H., Song, B., Park, O. J., Kim, Y."Pro‑apoptotic and migration‑suppressing potential of EGCG, and the involvement of AMPK in the p53‑mediated modulation of VEGF and MMP‑9 expression". Oncology Letters 6.5 (2013): 1346-1350.
Chicago
Park, S. Y., Jung, C. H., Song, B., Park, O. J., Kim, Y."Pro‑apoptotic and migration‑suppressing potential of EGCG, and the involvement of AMPK in the p53‑mediated modulation of VEGF and MMP‑9 expression". Oncology Letters 6, no. 5 (2013): 1346-1350. https://doi.org/10.3892/ol.2013.1533