A water-soluble extract from cultured medium of Ganoderma lucidum (Rei-shi) mycelia suppresses azoxymethane-induction of colon cancers in male F344 rats

  • Authors:
    • Huimei Lu
    • Eikai Kyo
    • Toshihiro Uesaka
    • Osamu Katoh
    • Hiromitsu Watanabe
  • View Affiliations

  • Published online on: March 1, 2003     https://doi.org/10.3892/or.10.2.375
  • Pages: 375-379
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Abstract

The present study was designed to investigate the protective effect of a dietary water-soluble extract from cultured medium of Ganoderma lucidum (Rei-shi or Mannentake) mycelia (designated as MAK) on the induction and development of azoxymethane (AOM)-induced colon tumors in male F344/Du Crj rats. A total of 80 animals were divided into five groups at six weeks of age, groups 2, 3 and 4 being given weekly subcutaneous injections of AOM (15 mg/kg body weight) for the initial 3 weeks to induce colon tumors. Rats in group 1 and 5 were injected with the vehicle, 0.9% (w/v) saline, following the same schedule. Rats in groups 1, 2, 3, 4 and 5 were fed MF, MF, 1.25% MAK, 2.5% MAK and 2.5% MAK diets, respectively, starting 1 week before AOM treatment and throughout the six-month experimental period. There were no significant differences in number of ACF, total AC and AC per site among groups 2 to 4, but the tumor incidence was significantly lower, and tumor size was smaller in group 4 (AOM + 2.5% MAK) than in group 2 (AOM + MF). Additionally, β-catenin positive tumor cell nuclei were significantly decreased in the MAK-fed rats (groups 3 and 4), which also demonstrated lowering of the PCNA labeling index and a shortened germinal region in the colon. The present results thus indicate that dietary MAK could act as a potent chemopreventive agent for colon carcinogenesis.

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March 2003
Volume 10 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Lu, H., Kyo, E., Uesaka, T., Katoh, O., & Watanabe, H. (2003). A water-soluble extract from cultured medium of Ganoderma lucidum (Rei-shi) mycelia suppresses azoxymethane-induction of colon cancers in male F344 rats. Oncology Reports, 10, 375-379. https://doi.org/10.3892/or.10.2.375
MLA
Lu, H., Kyo, E., Uesaka, T., Katoh, O., Watanabe, H."A water-soluble extract from cultured medium of Ganoderma lucidum (Rei-shi) mycelia suppresses azoxymethane-induction of colon cancers in male F344 rats". Oncology Reports 10.2 (2003): 375-379.
Chicago
Lu, H., Kyo, E., Uesaka, T., Katoh, O., Watanabe, H."A water-soluble extract from cultured medium of Ganoderma lucidum (Rei-shi) mycelia suppresses azoxymethane-induction of colon cancers in male F344 rats". Oncology Reports 10, no. 2 (2003): 375-379. https://doi.org/10.3892/or.10.2.375