Recovery of stromal stem cells in bone sarcoma patients after chemotherapy: Implication for cell-based therapy in bone defect reconstruction

  • Authors:
    • Amira Beccheroni
    • Enrico Lucarelli
    • Davide Donati
    • Luca Sangiorgi
    • Silvia Capponcelli
    • Marco Gorini
    • Annarosa Zambon Bertoja
    • Roberto Giardino
    • Mario Mercuri
    • Stefano Ferrari
    • Gaetano Bacci
    • Piero Picci
  • View Affiliations

  • Published online on: July 1, 2003     https://doi.org/10.3892/or.10.4.891
  • Pages: 891-896
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Abstract

Bone sarcomas, such as osteosarcoma (OS) or Ewing sarcoma (ES), frequently arise in the intramedullary region of long bones. Patients affected by bone sarcomas are treated with preoperative aggressive chemotherapy immediately after diagnosis. After chemotherapy, patients undergo surgery that frequently entails the excision of wide bone segments. If a large segment of the bone is lost (defined as a critical defect) the patient undergoes bone reconstruction. Because bone marrow derived stromal stem cells (SSC) offer great promise for cell-based regenerative medicine in bone reconstruction, we investigated whether SSC could be isolated from chemotherapy-treated bone sarcoma patients. We also investigated whether chemotherapy modified SSC differentiation capability. We studied 9 SSC derived from OS and ES patients that had undergone chemotherapy and 5 SSC derived from bone sarcoma patients that had not undergone chemotherapy. SSC could be obtained from all the patients analyzed regardless of whether the patients received chemotherapy or not. Our results also showed that post-chemotherapy SSC can be cultured and expanded ex vivo, these cells retained the ability to differentiate toward the osteogenic lineage in vitro. In conclusion, our results support that SSC cells can be obtained from bone sarcoma patients that undergo chemotherapy and suggest that SSC can be used for cell-based bone reconstruction techniques in bone sarcoma patients treated with preoperative chemotherapy.

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July-August 2003
Volume 10 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Beccheroni A, Lucarelli E, Donati D, Sangiorgi L, Capponcelli S, Gorini M, Zambon Bertoja A, Giardino R, Mercuri M, Ferrari S, Ferrari S, et al: Recovery of stromal stem cells in bone sarcoma patients after chemotherapy: Implication for cell-based therapy in bone defect reconstruction. Oncol Rep 10: 891-896, 2003
APA
Beccheroni, A., Lucarelli, E., Donati, D., Sangiorgi, L., Capponcelli, S., Gorini, M. ... Picci, P. (2003). Recovery of stromal stem cells in bone sarcoma patients after chemotherapy: Implication for cell-based therapy in bone defect reconstruction. Oncology Reports, 10, 891-896. https://doi.org/10.3892/or.10.4.891
MLA
Beccheroni, A., Lucarelli, E., Donati, D., Sangiorgi, L., Capponcelli, S., Gorini, M., Zambon Bertoja, A., Giardino, R., Mercuri, M., Ferrari, S., Bacci, G., Picci, P."Recovery of stromal stem cells in bone sarcoma patients after chemotherapy: Implication for cell-based therapy in bone defect reconstruction". Oncology Reports 10.4 (2003): 891-896.
Chicago
Beccheroni, A., Lucarelli, E., Donati, D., Sangiorgi, L., Capponcelli, S., Gorini, M., Zambon Bertoja, A., Giardino, R., Mercuri, M., Ferrari, S., Bacci, G., Picci, P."Recovery of stromal stem cells in bone sarcoma patients after chemotherapy: Implication for cell-based therapy in bone defect reconstruction". Oncology Reports 10, no. 4 (2003): 891-896. https://doi.org/10.3892/or.10.4.891