Differential involvement of mitochondria during ursolic acid-induced apoptotic process in HaCaT and M4Beu cells

  • Authors:
    • Raphaël Emmanuel Duval
    • Pierre-Olivier Harmand
    • Chantal Jayat-Vignoles
    • Jeanne Cook-Moreau
    • Aline Pinon
    • Christiane Delage
    • Alain Simon
  • View Affiliations

  • Published online on: January 1, 2008     https://doi.org/10.3892/or.19.1.145
  • Pages: 145-149
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Abstract

Ursolic acid (UA) is a pentacyclic triterpenoid compound which exists widely in nature and is known to have a pleitropic biological activity profile. For the last few decades, extensive work has been carried out to establish its biological activities and pharmacological actions. It is described as a promising chemopreventive agent with an antiproliferative effect on cancer cells that stems from its ability to induce apoptosis. We investigated and compared the role played by mitochondria during the apoptotic process induced by UA in human HaCaT-derived keratinotic cells and M4Beu human melanoma cells. In both cell lines, UA induced significant caspase-3 activation, the downstream central effector of apoptosis. Subsequent JC-1/TOTO-3 double staining clearly demonstrated that UA induces strong mitochondrial-transmembrane potential collapse in M4Beu cells, while mitochondria from HaCaT-treated cells remain largely unstimulated. This was confirmed by Western blot analysis, which revealed a Bax/Bcl-2-balance change in favor of Bax, the proapoptotic member, in UA-treated M4Beu cells. It can be concluded that UA induces apoptosis in M4Beu through the mitochondrial pathway, while other mechanisms are activated in the case of HaCaT cells.

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January 2008
Volume 19 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Duval RE, Harmand P, Jayat-Vignoles C, Cook-Moreau J, Pinon A, Delage C and Simon A: Differential involvement of mitochondria during ursolic acid-induced apoptotic process in HaCaT and M4Beu cells. Oncol Rep 19: 145-149, 2008
APA
Duval, R.E., Harmand, P., Jayat-Vignoles, C., Cook-Moreau, J., Pinon, A., Delage, C., & Simon, A. (2008). Differential involvement of mitochondria during ursolic acid-induced apoptotic process in HaCaT and M4Beu cells. Oncology Reports, 19, 145-149. https://doi.org/10.3892/or.19.1.145
MLA
Duval, R. E., Harmand, P., Jayat-Vignoles, C., Cook-Moreau, J., Pinon, A., Delage, C., Simon, A."Differential involvement of mitochondria during ursolic acid-induced apoptotic process in HaCaT and M4Beu cells". Oncology Reports 19.1 (2008): 145-149.
Chicago
Duval, R. E., Harmand, P., Jayat-Vignoles, C., Cook-Moreau, J., Pinon, A., Delage, C., Simon, A."Differential involvement of mitochondria during ursolic acid-induced apoptotic process in HaCaT and M4Beu cells". Oncology Reports 19, no. 1 (2008): 145-149. https://doi.org/10.3892/or.19.1.145