2-DE analysis of breast cancer cell lines 1833 and 4175 with distinct metastatic organ-specific potentials: Comparison with parental cell line MDA-MB-231

  • Authors:
    • Irena Selicharova
    • Miloslav Sanda
    • Jana Mladkova
    • Sujata Saraswat Ohri
    • Aruna Vashishta
    • Martin Fusek
    • Jiri Jiracek
    • Vaclav Vetvicka
  • View Affiliations

  • Published online on: May 1, 2008     https://doi.org/10.3892/or.19.5.1237
  • Pages: 1237-1244
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Abstract

Human MDA-MB-231 derived breast cancer cell lines 1833 and 4175 have different metastatic potentials in terms of their tissue tropisms and aggressiveness. Cell line 1833 is specifically metastatic to the bone. The highly aggressive cell line 4175 is specific to the lung. We performed 2-DE analysis of the cell lines. We found 16 significantly changed protein spots, 14 protein spots were identified. Expression of cathepsin D, triosephosphate isomerase, phosphoglycerate kinase 1, heme binding protein 1 and annexin 2 could be correlated with the in vitro aggressiveness of the respective cell lines. Interstitial collagenase and dimethylargininase 2 were exclusive to the cell line 1833 and might contribute to its bone specificity. Serpin B9, cathepsin B chain b, galectin 3 and HSP 27 were changed in the lung specific cell line 4175. The possible contribution of identified proteins to differences in metastatic behavior of the cell lines is discussed.

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May 2008
Volume 19 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Selicharova, I., Sanda, M., Mladkova, J., Ohri, S.S., Vashishta, A., Fusek, M. ... Vetvicka, V. (2008). 2-DE analysis of breast cancer cell lines 1833 and 4175 with distinct metastatic organ-specific potentials: Comparison with parental cell line MDA-MB-231. Oncology Reports, 19, 1237-1244. https://doi.org/10.3892/or.19.5.1237
MLA
Selicharova, I., Sanda, M., Mladkova, J., Ohri, S. S., Vashishta, A., Fusek, M., Jiracek, J., Vetvicka, V."2-DE analysis of breast cancer cell lines 1833 and 4175 with distinct metastatic organ-specific potentials: Comparison with parental cell line MDA-MB-231". Oncology Reports 19.5 (2008): 1237-1244.
Chicago
Selicharova, I., Sanda, M., Mladkova, J., Ohri, S. S., Vashishta, A., Fusek, M., Jiracek, J., Vetvicka, V."2-DE analysis of breast cancer cell lines 1833 and 4175 with distinct metastatic organ-specific potentials: Comparison with parental cell line MDA-MB-231". Oncology Reports 19, no. 5 (2008): 1237-1244. https://doi.org/10.3892/or.19.5.1237