Modulation of intestinal environment by prebiotic germinated barley foodstuff prevents chemo-induced colonic carcinogenesis in rats

  • Authors:
    • Osamu Kanauchi
    • Keiichi Mitsuyama
    • Akira Andoh
    • Toshihiko Iwanaga
  • View Affiliations

  • Published online on: October 1, 2008     https://doi.org/10.3892/or_00000076
  • Pages: 793-801
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Abstract

Butyrate was shown to have a preventive effect on colon cancer in vivo. Germinated barley foodstuff (GBF) was in a prebiotic stage and had the potency to attenuate mucosal inflammation and to increase fecal butyrate production in colitis. This study aimed to determine whether the GBF treatment in a colon cancer model had the potency to suppress colon cancer. After a pre-feeding of either a control or a GBF diet for two weeks, male F344 rats received subcutaneous injections of azoxymethane twice, at a dose level of 15 mg/kg body weight. The injections were administered once a week for 2 weeks (n=10/group). Four weeks after that, the number of aberrant crypt foci (ACF) and heat shock protein (HSP) 25-positive cells in colonic mucosa were observed histologically. The mRNA level of slc5a8 was evaluated by in situ hybridization. Colonic mucosal β-catenin was determined by Western blotting. Cecal short chain fatty acids, β-glucosidase and β-glucuronidase were also determined. The results showed that GBF treatment significantly decreased the number of ACF and β-catenin formations in the colonic mucosa. GBF significantly increased the production of slc5a8, which is a tumor suppressor gene, as well as the cecal butyrate content and β-glucosidase activity. β-glucuronidase activity remained at the same level in GBF and control subjects. The number of HSP25-positive cells in GBF was higher than that in the control group, although it did not reach significant difference. In conclusion, GBF showed anti-tumorigenicity in the AOM rat model. Changes in the colonic environment featured through the increase of butyrate production were found. Although a more detailed study is required, this study showed the promising anti-neoplastic effects of prebiotic treatment.

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October 2008
Volume 20 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kanauchi O, Mitsuyama K, Andoh A and Iwanaga T: Modulation of intestinal environment by prebiotic germinated barley foodstuff prevents chemo-induced colonic carcinogenesis in rats. Oncol Rep 20: 793-801, 2008
APA
Kanauchi, O., Mitsuyama, K., Andoh, A., & Iwanaga, T. (2008). Modulation of intestinal environment by prebiotic germinated barley foodstuff prevents chemo-induced colonic carcinogenesis in rats. Oncology Reports, 20, 793-801. https://doi.org/10.3892/or_00000076
MLA
Kanauchi, O., Mitsuyama, K., Andoh, A., Iwanaga, T."Modulation of intestinal environment by prebiotic germinated barley foodstuff prevents chemo-induced colonic carcinogenesis in rats". Oncology Reports 20.4 (2008): 793-801.
Chicago
Kanauchi, O., Mitsuyama, K., Andoh, A., Iwanaga, T."Modulation of intestinal environment by prebiotic germinated barley foodstuff prevents chemo-induced colonic carcinogenesis in rats". Oncology Reports 20, no. 4 (2008): 793-801. https://doi.org/10.3892/or_00000076