Expression of 14-3-3σ in cervical squamous cell carcinomas: Relationship with clinical outcome

  • Authors:
    • Ruth Holm
    • Tayeba Ali
    • Debbie H. Svendsrud
    • Jahn M. Nesland
    • Gunnar B. Kristensen
    • Heidi Lyng
  • View Affiliations

  • Published online on: July 1, 2009     https://doi.org/10.3892/or_00000399
  • Pages: 11-15
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Abstract

14-3-3 sigma (σ) sequesters the cdc2-cyclin B1 complex in the cytoplasm resulting in G2 arrest. Inactivation and reduced expression of 14-3-3σ have been reported in a varity of cancers. In the present study, we investigated the expression of 14-3-3σ in a series of 297 cervical squamous cell carcinoma (SCC) to clarify the prognostic value. Using immunohistochemical methods we found high levels of 14-3-3σ protein in cytoplasm of 143 (48.1%), in nucleus of 113 (38.0%) and in both cytoplasm and nucleus of 147 (49.5%) cases, whereas, low levels were present in cytoplasm of 154 (51.9%), in nucleus of 184 (62.0%) and in both cytoplasm and nucleus of 150 (50.5%) cases. Levels of 14-3-3σ mRNA measured by reverse-transcription polymerase chain reaction (RT-PCR) and 14-3-3σ protein were not significant associated. 14-3-3σ expression in cytoplasm, nuclear and cytoplasm/nuclear were not significantly correlated to disease-specific survival or disease-free survival. In conclusion, reduced expression of 14-3-3σ protein in the cytoplasm and shuttle of 14-3-3σ protein into the nucleus in a relatively high number of cases indicate that 14-3-3σ may be important in the carcinogenesis of cervical SCCs by two different mechanisms; reduction and nuclear translocation of 14-3-3σ protein. Furthermore, the non-significant correlation between expression levels of 14-3-3σ mRNA and protein support a post-transcriptional regulation in cervical SCCs. The protein has no prognostic value in cervical cancers.

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July 2009
Volume 22 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Holm, R., Ali, T., Svendsrud, D.H., Nesland, J.M., Kristensen, G.B., & Lyng, H. (2009). Expression of 14-3-3σ in cervical squamous cell carcinomas: Relationship with clinical outcome. Oncology Reports, 22, 11-15. https://doi.org/10.3892/or_00000399
MLA
Holm, R., Ali, T., Svendsrud, D. H., Nesland, J. M., Kristensen, G. B., Lyng, H."Expression of 14-3-3σ in cervical squamous cell carcinomas: Relationship with clinical outcome". Oncology Reports 22.1 (2009): 11-15.
Chicago
Holm, R., Ali, T., Svendsrud, D. H., Nesland, J. M., Kristensen, G. B., Lyng, H."Expression of 14-3-3σ in cervical squamous cell carcinomas: Relationship with clinical outcome". Oncology Reports 22, no. 1 (2009): 11-15. https://doi.org/10.3892/or_00000399