The effect of MG132, a proteasome inhibitor on HeLa cells in relation to cell growth, reactive oxygen species and GSH

  • Authors:
    • Yong Hwan Han
    • Hwa Jin Moon
    • Bo Ra You
    • Woo Hyun Park
  • View Affiliations

  • Published online on: July 1, 2009     https://doi.org/10.3892/or_00000427
  • Pages: 215-221
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Abstract

MG132 (carbobenzoxy-Leu-Leu-leucinal) is a peptide aldehyde, which effectively blocks the proteolytic activity of the 26S proteasome complex. We evaluated the effects of MG132 on the growth of human cervix cancer HeLa cells in relation to the cell growth, reactive oxygen species (ROS) and glutathione (GSH) levels. Dose-dependent inhibition of cell growth was observed in HeLa cells with an IC50 of approximately 5 µM MG132 for 24 h. DNA flow cytometric analysis indicated that treatment with MG132 induced S, G2-M or non-specific phase arrests of the cell cycle dose-dependently. Treatment with MG132 induced apoptosis in a dose-dependent manner, as evidenced by sub-G1 cells and annexin V staining cells. Treatment with MG132 also induced the loss of mitochondrial membrane potential in HeLa cells. The intracellular ROS levels including O2·− were significantly increased in MG132-treated cells. Furthermore, the depletion of intracellular GSH content was observed in cells treated with MG132. In conclusion, MG132 inhibited the growth of HeLa cells via inducing the cell cycle arrest as well as triggering apoptosis. The changes of ROS and GSH by MG132 were closely related to apoptosis in HeLa cells.

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July 2009
Volume 22 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Han, Y.H., Moon, H.J., You, B.R., & Park, W.H. (2009). The effect of MG132, a proteasome inhibitor on HeLa cells in relation to cell growth, reactive oxygen species and GSH. Oncology Reports, 22, 215-221. https://doi.org/10.3892/or_00000427
MLA
Han, Y. H., Moon, H. J., You, B. R., Park, W. H."The effect of MG132, a proteasome inhibitor on HeLa cells in relation to cell growth, reactive oxygen species and GSH". Oncology Reports 22.1 (2009): 215-221.
Chicago
Han, Y. H., Moon, H. J., You, B. R., Park, W. H."The effect of MG132, a proteasome inhibitor on HeLa cells in relation to cell growth, reactive oxygen species and GSH". Oncology Reports 22, no. 1 (2009): 215-221. https://doi.org/10.3892/or_00000427