Open Access

Anti-tumor effect of cimetidine via inhibiting angiogenesis factors in N-butyl-N-(4-hydroxybutyl) nitrosamine-induced mouse and rat bladder carcinogenesis

  • Authors:
    • Yoshitomo Chihara
    • Kiyohide Fujimoto
    • Makito Miyake
    • Yoshio Hiasa
    • Yoshihiko Hirao
  • View Affiliations

  • Published online on: July 1, 2009     https://doi.org/10.3892/or_00000401
  • Pages:23-28
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Abstract

The aim of this study was to assess the anti-tumor effect and mechanisms of cimetidine in N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN)-induced bladder carcinogenesis model. Sixty-three male BALB/c mice and 67 male Wister rats were treated with BBN and cimetidine to examine the anti-tumor effect of cimetidine. Immunohistochemistry (IHC) of vascular endothelial growth factor (VEGF), platelet-derived endothelial growth factor (PDECGF), and E-selectin were examined to compare their expression in the tumor tissues. In mice, the tumor growth was reduced by cimetidine (p=0.011). The expression of PDECGF was reduced in the cimetidine-treated group (p=0.016). In rats, treatment of cimetidine reduced tumor growth (p=0.0001). Moreover, the expression of VEGF and PDECGF was reduced (p=0.02 and <0.001, respectively). The expression of E-selectin did not correlate with the tumor growth in either mice or rats. In mice, long-term cimetidine treatment proved very effective for inhibiting the tumor growth, but in rats, BBN after treatment with cimetidine showed the least tumor growth-inhibitory effect. In conclusion, cimetidine may have an inhibitory effect on tumor growth in bladder carcinogenesis via reducing the expression of angiogenesis factors including VEGF and PDECGF.

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July 2009
Volume 22 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Chihara, Y., Fujimoto, K., Miyake, M., Hiasa, Y., & Hirao, Y. (2009). Anti-tumor effect of cimetidine via inhibiting angiogenesis factors in N-butyl-N-(4-hydroxybutyl) nitrosamine-induced mouse and rat bladder carcinogenesis. Oncology Reports, 22, 23-28. https://doi.org/10.3892/or_00000401
MLA
Chihara, Y., Fujimoto, K., Miyake, M., Hiasa, Y., Hirao, Y."Anti-tumor effect of cimetidine via inhibiting angiogenesis factors in N-butyl-N-(4-hydroxybutyl) nitrosamine-induced mouse and rat bladder carcinogenesis". Oncology Reports 22.1 (2009): 23-28.
Chicago
Chihara, Y., Fujimoto, K., Miyake, M., Hiasa, Y., Hirao, Y."Anti-tumor effect of cimetidine via inhibiting angiogenesis factors in N-butyl-N-(4-hydroxybutyl) nitrosamine-induced mouse and rat bladder carcinogenesis". Oncology Reports 22, no. 1 (2009): 23-28. https://doi.org/10.3892/or_00000401