Open Access

Antitumor activity of bevacizumab in combination with capecitabine and oxaliplatin in human colorectal cancer xenograft models

  • Authors:
    • Mieko Yanagisawa
    • Kaori Fujimoto-Ouchi
    • Keigo Yorozu
    • Yoriko Yamashita
    • Kazushige Mori
  • View Affiliations

  • Published online on: August 1, 2009     https://doi.org/10.3892/or_00000430
  • Pages: 241-247
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Abstract

To understand the mechanisms of the effects of combination treatments, we established animal models showing antitumor activity of bevacizumab as a monotherapy and in combination with capecitabine or capecitabine and oxaliplatin and measured thymidine phosphorylase (TP) and vascular endothelial growth factor (VEGF) levels. Tumor-inoculated nude mice were treated with bevacizumab, capecitabine, and oxaliplatin, alone or in combination, after tumor growth was confirmed and volume and microvessel density (MVD) in tumors were evaluated. Levels of TP and VEGF in the tumor were examined by ELISA. Bevacizumab showed significant antitumor activity as a monotherapy in three xenograft models (COL-16-JCK, COLO 205 and CXF280). The MVD in tumor tissues treated with bevacizumab was lower than that of the control. Antitumor activity of bevacizumab in combination with capecitabine was significantly higher than that of each agent alone (COL-16-JCK, COLO 205). Furthermore, the antitumor activity of bevacizumab in combination with capecitabine + oxaliplatin was significantly superior to that of capecitabine + oxaliplatin (COL-16-JCK). TP and VEGF levels were not increased by bevacizumab or capecitabine, respectively, suggesting there are other potentially efficacious mechanisms involved. In the present study we established human colorectal cancer xenograft models which reflect the efficacy of clinical combination therapies, capecitabine + bevacizumab and capecitabine + oxaliplatin + bevacizumab. We will further investigate the mechanisms of the combination therapies using these models.

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August 2009
Volume 22 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Yanagisawa, M., Fujimoto-Ouchi, K., Yorozu, K., Yamashita, Y., & Mori, K. (2009). Antitumor activity of bevacizumab in combination with capecitabine and oxaliplatin in human colorectal cancer xenograft models. Oncology Reports, 22, 241-247. https://doi.org/10.3892/or_00000430
MLA
Yanagisawa, M., Fujimoto-Ouchi, K., Yorozu, K., Yamashita, Y., Mori, K."Antitumor activity of bevacizumab in combination with capecitabine and oxaliplatin in human colorectal cancer xenograft models". Oncology Reports 22.2 (2009): 241-247.
Chicago
Yanagisawa, M., Fujimoto-Ouchi, K., Yorozu, K., Yamashita, Y., Mori, K."Antitumor activity of bevacizumab in combination with capecitabine and oxaliplatin in human colorectal cancer xenograft models". Oncology Reports 22, no. 2 (2009): 241-247. https://doi.org/10.3892/or_00000430