Inhibition of matrix metalloproteinase expression by menatetrenone, a vitamin K2 analogue

  • Authors:
    • Yasushi Ide
    • Hao Zhang
    • Hiroshi Hamajima
    • Yasunori Kawaguchi
    • Yuichiro Eguchi
    • Toshihiko Mizuta
    • Kyosuke Yamamoto
    • Kazuma Fujimoto
    • Iwata Ozaki
  • View Affiliations

  • Published online on: Tuesday, September 1, 2009
  • Pages:599-604 DOI: 10.3892/or_00000478
0

Abstract

Vitamin K2 (VK2) has been shown to have a potent anti-tumor effect against several cancer types including hepatocellular carcinoma (HCC), but the mechanisms remain to be elucidated. Matrix metalloproteinase (MMP) plays an important role in the invasion and metastasis of cancer cells, but it is not known whether VK2 regulates the expression of MMPs. Human HCC cell lines were treated with VK2 combined with 12-O-tetradecanoyl phorbol-13 acetate (TPA) and the expression of MMPs was examined by reporter gene assay, RT-PCR and Western blotting. VK2 inhibited the basal and TPA-induced expression of MMP-1, -3 and -7 at the transcriptional, mRNA and protein levels in a dose-dependent manner. VK2 also inhibited the TPA-induced activation of NF-κB and AP-1 activity. The inhibitors against NF-κB and mitogen-activated protein kinases (MAP kinase) including ERK and JNK pathways suppressed TPA-induced luciferase activity of MMP-1, -3 and -7 promoters. These data suggest that VK2 inhibits MMP expression by suppressing NF-κB and MAP kinase activity and might be potentially useful in the treatment of HCC.

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September 2009
Volume 22 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

2015 Impact Factor: 2.486
Ranked #31/211 Oncology
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APA
Ide, Y., Zhang, H., Hamajima, H., Kawaguchi, Y., Eguchi, Y., Mizuta, T. ... Ozaki, I. (2009). Inhibition of matrix metalloproteinase expression by menatetrenone, a vitamin K2 analogue. Oncology Reports, 22, 599-604. http://dx.doi.org/10.3892/or_00000478
MLA
Ide, Y., Zhang, H., Hamajima, H., Kawaguchi, Y., Eguchi, Y., Mizuta, T., Yamamoto, K., Fujimoto, K., Ozaki, I."Inhibition of matrix metalloproteinase expression by menatetrenone, a vitamin K2 analogue". Oncology Reports 22.3 (2009): 599-604.
Chicago
Ide, Y., Zhang, H., Hamajima, H., Kawaguchi, Y., Eguchi, Y., Mizuta, T., Yamamoto, K., Fujimoto, K., Ozaki, I."Inhibition of matrix metalloproteinase expression by menatetrenone, a vitamin K2 analogue". Oncology Reports 22, no. 3 (2009): 599-604. http://dx.doi.org/10.3892/or_00000478