Open Access

Modulation of MMP-2 and MMP-9 by cytokines, mitogens and inhibitors in lung cancer and malignant mesothelioma cell lines

  • Authors:
    • M. W. Roomi
    • Julio C. Monterrey
    • Tatiana Kalinovsky
    • Aleksandra Niedzwiecki
    • Matthias Rath
  • View Affiliations

  • Published online on: December 1, 2009     https://doi.org/10.3892/or_00000566
  • Pages: 1283-1291
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Abstract

Matrix metalloproteinases (MMPs) secreted by lung cancer (LC) and malignant mesothelioma (MM), especially MMP-2 and MMP-9, play crucial roles in tumor invasion and metastasis. We examined the effect of cytokines, mitogens and inhibitors on MMP-2 and MMP-9 expression in LC and MM cell lines. Human LC (A-549) and MM (MSTO-211H) cell lines were cultured in appropriate media. At near confluence, the cells were washed with PBS and incubated in serum-free medium with various concentrations of several cytokines, mitogens and inhibitors. After 24 h the media were removed and analyzed for MMP-2 and MMP-9 by gelatinase zymography and quantitated by densitometry. LC expressed MMP-2 whereas MM expressed MMP-2 and MMP-9. TNF-α, IL-1β, LPS and PMA, stimulated MMP-2 in LC and inhibited MMP-2 in MM, but had no effect on MMP-9. Doxycycline, EGCG and NM inhibited MMP-2 and MMP-9 expression, in both cell lines. Actinomycin-D, cyclohexamide, retinoic acid and dexamethasone inhibited MMP-2 in both cancer cell lines and inhibited MMP-9 in MM. Our results show that cytokines and inhibitors have an up- or down-regulatory effect on MMP-2 and MMP-9 expression in LC and MM, suggesting the clinical value of targeting these proteases for management of LC and MM and their pathogenesis.

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December 2009
Volume 22 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Roomi, M.W., Monterrey, J.C., Kalinovsky, T., Niedzwiecki, A., & Rath, M. (2009). Modulation of MMP-2 and MMP-9 by cytokines, mitogens and inhibitors in lung cancer and malignant mesothelioma cell lines. Oncology Reports, 22, 1283-1291. https://doi.org/10.3892/or_00000566
MLA
Roomi, M. W., Monterrey, J. C., Kalinovsky, T., Niedzwiecki, A., Rath, M."Modulation of MMP-2 and MMP-9 by cytokines, mitogens and inhibitors in lung cancer and malignant mesothelioma cell lines". Oncology Reports 22.6 (2009): 1283-1291.
Chicago
Roomi, M. W., Monterrey, J. C., Kalinovsky, T., Niedzwiecki, A., Rath, M."Modulation of MMP-2 and MMP-9 by cytokines, mitogens and inhibitors in lung cancer and malignant mesothelioma cell lines". Oncology Reports 22, no. 6 (2009): 1283-1291. https://doi.org/10.3892/or_00000566