Depletion of PI3K p85α induces cell cycle arrest and apoptosis in colorectal cancer cells

  • Authors:
    • Yan Sun
    • Shiyi Zhao
    • Hua Tian
    • Xiaoyun Xie
    • Faman Xiao
    • Kang Li
    • Yugang Song
  • View Affiliations

  • Published online on: December 1, 2009     https://doi.org/10.3892/or_00000585
  • Pages: 1435-1441
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Abstract

Colorectal cancer is one of the most common malignancies in the world. Overactivity of phosphatidylinositol 3-kinase (PI3K) is frequently detected in colorectal carcinoma. PI3K signaling plays a pivotal role in intracellular signal transduction pathways involved in cell growth, cellular transformation, and tumorigenesis. To specifically inhibit PI3K activity in colorectal cancer cells, we constructed a siRNA against the PI3K regulatory subunit p85α and transfected it into LoVo and SW480 cells. In the present study, treatment of colorectal cancer cells with PI3K p85α-specific siRNA inhibited cell proliferation, induced G1 phase cell cycle arrest and sensitized colorectal cancer cells to 5-FU-induced apoptosis. Furthermore, depletion of PI3K p85α resulted in significant activation of three Forkhead box class O (FoxO) transcription factors, which inhibited the expression of cyclin D1, cdk4 and induced expression of p27/Kip1. Activation of FoxO transcription factors also increased the expression of FasL. Thus, our results indicate that siRNA-mediated gene silencing of PI3K p85α may be a useful therapeutic strategy for colorectal carcinoma.

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December 2009
Volume 22 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Sun Y, Zhao S, Tian H, Xie X, Xiao F, Li K and Song Y: Depletion of PI3K p85α induces cell cycle arrest and apoptosis in colorectal cancer cells . Oncol Rep 22: 1435-1441, 2009
APA
Sun, Y., Zhao, S., Tian, H., Xie, X., Xiao, F., Li, K., & Song, Y. (2009). Depletion of PI3K p85α induces cell cycle arrest and apoptosis in colorectal cancer cells . Oncology Reports, 22, 1435-1441. https://doi.org/10.3892/or_00000585
MLA
Sun, Y., Zhao, S., Tian, H., Xie, X., Xiao, F., Li, K., Song, Y."Depletion of PI3K p85α induces cell cycle arrest and apoptosis in colorectal cancer cells ". Oncology Reports 22.6 (2009): 1435-1441.
Chicago
Sun, Y., Zhao, S., Tian, H., Xie, X., Xiao, F., Li, K., Song, Y."Depletion of PI3K p85α induces cell cycle arrest and apoptosis in colorectal cancer cells ". Oncology Reports 22, no. 6 (2009): 1435-1441. https://doi.org/10.3892/or_00000585