miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells

  • Authors:
    • Shu-Yang Xie
    • You-Jie Li
    • Ping-Yu Wang
    • Fei Jiao
    • Shuai Zhang
    • Wen-Juan Zhang
  • View Affiliations

  • Published online on: June 1, 2010     https://doi.org/10.3892/or_00000813
  • Pages:1693-1700
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Abstract

To explore the mechanism of apoptosis induced by cisplatin, the expression of microRNAs (miRNAs) and regulating genes in K562 cells was analyzed using reverse transcription PCR, quantitative real-time PCR and enzyme-linked immunosorbent assays. Our results showed that miR-16, miR-34a-c, miR-17-5p and miR-125 were up-regulated, and their associated oncogenes (BCL2, E2F1 and E2F3, respectively) were down-regulated after cisplatin treatment. We also showed that miR-106 and miR-150 were down-regulated while their target genes (RB1 and P53, respectively) were up-regulated after cisplatin treatment. Moreover, miR-16, miR-34a-c and miR-17-5p proved to be upstream factors, regulating the expression of BCL2, E2F1 and E2F3, respectively. The oncogene E2F3 was down-regulated when RB1 expression was increased after treatment with antisense oligonucleotides (ASO). Similarly, BCL2 and E2F3 were down-regulated when P53 expression was elevated by ASO treatment. The study demonstrated that cisplatin induces K562 cells to apoptosis by reducing miR-106 which up-regulates RB1 or by inhibiting miR-150 which increases P53 expression.

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June 2010
Volume 23 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

2016 Impact Factor: 2.662
Ranked #31/217 Oncology
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APA
Xie, S., Li, Y., Wang, P., Jiao, F., Zhang, S., & Zhang, W. (2010). miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells . Oncology Reports, 23, 1693-1700. https://doi.org/10.3892/or_00000813
MLA
Xie, S., Li, Y., Wang, P., Jiao, F., Zhang, S., Zhang, W."miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells ". Oncology Reports 23.6 (2010): 1693-1700.
Chicago
Xie, S., Li, Y., Wang, P., Jiao, F., Zhang, S., Zhang, W."miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells ". Oncology Reports 23, no. 6 (2010): 1693-1700. https://doi.org/10.3892/or_00000813