Apoptotic activity of a new jasmonate analogue is associated with its induction of DNA damage

  • Authors:
    • Jing Zhao
    • Saeromi Kang
    • Xin Zhang
    • Song You
    • Jang-Su Park
    • Jee H. Jung
    • Dong-Kyoo Kim
  • View Affiliations

  • Published online on: September 1, 2010     https://doi.org/10.3892/or_00000920
  • Pages:771-777
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Abstract

The current study was undertaken to investigate the effects of methyl 5-chloro-4,5-didehydrojasmonate (J7), an analogue of methyl jasmonate, on the in vitro growth of human cervical carcinoma HeLa cells. Significantly decreased rates of viability (IC50 ≈15 µM) as well as evidence of apoptosis were observed with J7. Cell morphological changes observed under light microscopy confirmed apoptosis occurrence. Furthermore, the results from Annexin V-FITC/PI double staining and the cell cycle arrest assay indicated that J7 induced earlier apoptosis of HeLa cells. J7 also reduced the expression of Bcl-2 and subsequent activation of a protease cascade involving caspase-9 and -3 by Western blot assay was observed. We also found that J7 was able to induce DNA damage. These findings suggest that J7 induces HeLa cell apoptosis by activation of caspase pathway and the apoptotic effect is associated with DNA damage. Therefore, J7 may be a candidate compound to be developed into an anticancer agent.

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September 2010
Volume 24 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

2016 Impact Factor: 2.662
Ranked #31/217 Oncology
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APA
Zhao, J., Kang, S., Zhang, X., You, S., Park, J., Jung, J.H., & Kim, D. (2010). Apoptotic activity of a new jasmonate analogue is associated with its induction of DNA damage . Oncology Reports, 24, 771-777. https://doi.org/10.3892/or_00000920
MLA
Zhao, J., Kang, S., Zhang, X., You, S., Park, J., Jung, J. H., Kim, D."Apoptotic activity of a new jasmonate analogue is associated with its induction of DNA damage ". Oncology Reports 24.3 (2010): 771-777.
Chicago
Zhao, J., Kang, S., Zhang, X., You, S., Park, J., Jung, J. H., Kim, D."Apoptotic activity of a new jasmonate analogue is associated with its induction of DNA damage ". Oncology Reports 24, no. 3 (2010): 771-777. https://doi.org/10.3892/or_00000920