Curcumin promotes apoptosis in human lung adenocarcinoma cells through miR-186* signaling pathway

  • Authors:
    • Jian Zhang
    • Yongping Du
    • Changgui Wu
    • Xinling Ren
    • Xinyu Ti
    • Jieran Shi
    • Feng Zhao
    • Hong Yin
  • View Affiliations

  • Published online on: November 1, 2010     https://doi.org/10.3892/or_00000975
  • Pages: 1217-1223
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Abstract

Lung cancer is one of the leading causes of cancer-related death worldwide. Curcumin has been reported to have an antitumor effect by inducing apoptosis and suppressing growth of tumor cells. However, the mechanism by which curcumin exerts its anti-cancer effect needs further research. The purpose of the present study was to identify a miRNA-mediated mechanism which plays a role in the anti-cancer effects of curcumin. Alterations in miRNA expression were seen in curcumin-treated A549 cells, including significant downregulation of miRNA-186* expression by microarray analysis and real-time PCR. The miRNA-186* functions by overexpression or inhibition were investigated using biological assays in A549 cells. Additionally, caspase-10 was identified as a target of miRNA-186* using dual luciferase reporter assays and western blot analysis. These results demonstrate that curcumin induces A549 cell apoptosis through a miRNA pathway. Also, miRNA-186* could serve as a potential gene therapy target in curcumin treatment. furthermore, caspase-10 was shown to be a target of miR-186* regulation.

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November 2010
Volume 24 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang J, Du Y, Wu C, Ren X, Ti X, Shi J, Zhao F and Yin H: Curcumin promotes apoptosis in human lung adenocarcinoma cells through miR-186* signaling pathway . Oncol Rep 24: 1217-1223, 2010
APA
Zhang, J., Du, Y., Wu, C., Ren, X., Ti, X., Shi, J. ... Yin, H. (2010). Curcumin promotes apoptosis in human lung adenocarcinoma cells through miR-186* signaling pathway . Oncology Reports, 24, 1217-1223. https://doi.org/10.3892/or_00000975
MLA
Zhang, J., Du, Y., Wu, C., Ren, X., Ti, X., Shi, J., Zhao, F., Yin, H."Curcumin promotes apoptosis in human lung adenocarcinoma cells through miR-186* signaling pathway ". Oncology Reports 24.5 (2010): 1217-1223.
Chicago
Zhang, J., Du, Y., Wu, C., Ren, X., Ti, X., Shi, J., Zhao, F., Yin, H."Curcumin promotes apoptosis in human lung adenocarcinoma cells through miR-186* signaling pathway ". Oncology Reports 24, no. 5 (2010): 1217-1223. https://doi.org/10.3892/or_00000975