Antitumor effects of nano-bubble hydrogen-dissolved water are enhanced by coexistent platinum colloid and the combined hyperthermia with apoptosis-like cell death

  • Authors:
    • Ryoko Asada
    • Katsuhiro Kageyama
    • Hiroshi Tanaka
    • Hisakazu Matsui
    • Masatsugu Kimura
    • Yasukazu Saitoh
    • Nobuhiko Miwa
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/or_00001006
  • Pages: 1463-1470
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Abstract

In order to erase reactive oxygen species (ROS) related with the proliferation of tumor cells by reducing activity of hydrogen, we developed functional water containing nano-bubbles (diameters: <900 nm for 71%/population) hydrogen of 1.1-1.5 ppm (the theoretical maximum: 1.6 ppm) with a reducing ability (an oxidation-reduction potential -650 mV, normal water: +100-200 mV) using a microporous-filter hydrogen-jetting device. We showed that hydrogen water erased ROS indispensable for tumor cell growth by ESR/spin trap, the redox indicator CDCFH-DA assay, and was cytotoxic to Ehrlich ascites tumor cells as assessed by WST-8 assay, crystal violet dye stain and scanning electron microscopy, after 24-h or 48-h incubation sequent to warming at 37°C or 42°C. Hydrogen water supplemented with platinum colloid (0.3 ppm Pt in 4% polyvinylpyrrolidone) had more antitumor activity than hydrogen water alone, mineral water alone (15.6%), hydrogen water plus mineral water, or platinum colloid alone as observed by decreased cell numbers, cell shrinkage and pycnosis (nuclear condensation)/karyorrhexis (nuclear fragmentation) indicative of apoptosis, together with cell deformation and disappearance of microvilli on the membrane surface. These antitumor effects were promoted by combination with hyperthermia at 42°C. Thus, the nano-bubble hydrogen water with platinum colloid is potent as an anti-tumor agent.

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December 2010
Volume 24 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Asada, R., Kageyama, K., Tanaka, H., Matsui, H., Kimura, M., Saitoh, Y., & Miwa, N. (2010). Antitumor effects of nano-bubble hydrogen-dissolved water are enhanced by coexistent platinum colloid and the combined hyperthermia with apoptosis-like cell death . Oncology Reports, 24, 1463-1470. https://doi.org/10.3892/or_00001006
MLA
Asada, R., Kageyama, K., Tanaka, H., Matsui, H., Kimura, M., Saitoh, Y., Miwa, N." Antitumor effects of nano-bubble hydrogen-dissolved water are enhanced by coexistent platinum colloid and the combined hyperthermia with apoptosis-like cell death ". Oncology Reports 24.6 (2010): 1463-1470.
Chicago
Asada, R., Kageyama, K., Tanaka, H., Matsui, H., Kimura, M., Saitoh, Y., Miwa, N." Antitumor effects of nano-bubble hydrogen-dissolved water are enhanced by coexistent platinum colloid and the combined hyperthermia with apoptosis-like cell death ". Oncology Reports 24, no. 6 (2010): 1463-1470. https://doi.org/10.3892/or_00001006