Genetic pathways of multiple esophageal squamous cell carcinomas

  • Authors:
    • Takayasu Kuwabara
    • Toru Hiyama
    • Shinji Tanaka
    • Masaharu Yoshihara
    • Koji Arihiro
    • Kazuaki Chayama
  • View Affiliations

  • Published online on: December 14, 2010     https://doi.org/10.3892/or.2010.1110
  • Pages:453-459
Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

Whether multiple esophageal squamous cell carcinomas (SCCs) in a patient develop through an identical genetic pathway is still unclear. We examined multiple esophageal SCCs for alterations of p53, p16, IRF and mitochondrial DNA (mtDNA) and microsatellite instability (MSI). Thirty patients with multiple superficial esophageal SCCs, 23 with double lesions and 7 with triple lesions, were enrolled. Loss of heterozygosity (LOH) of p53 (TP53), p16 (D9S171), IRF (IRF) and other microsatellite loci including D1S191, D17S858, D18S58 and D18S61 of the tumors was examined by microsatellite assay. Mutations of p16 and mtDNA were examined with PCR single-strand conformation polymorphism (SSCP) analysis. LOH of p53, p16 and IRF were detected in 16 of 50 (32%), 5 of 38 (13%) and 5 of 48 (10%) tumors, respectively. Mutations of p16 were detected in 4 of 67 (6%) tumors. Six of 67 (9%) tumors had mtDNA alterations and none of the tumors showed high-frequency MSI. All 30 patients showed one or more gene alterations in one or more genetic loci. Discordant genetic patterns among individual lesions within a patient were observed in 28 of the 30 (93%) patients. The most discordant locus was TP53, present in 11 of 29 (38%) informative cases, followed by D18S61, present in 11 of 30 (37%) informative cases. These results suggest that the genetic pathways of multiple esophageal SCCs may differ even within the same patient.

Related Articles

Journal Cover

February 2011
Volume 25 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Kuwabara, T., Hiyama, T., Tanaka, S., Yoshihara, M., Arihiro, K., & Chayama, K. (2011). Genetic pathways of multiple esophageal squamous cell carcinomas. Oncology Reports, 25, 453-459. https://doi.org/10.3892/or.2010.1110
MLA
Kuwabara, T., Hiyama, T., Tanaka, S., Yoshihara, M., Arihiro, K., Chayama, K."Genetic pathways of multiple esophageal squamous cell carcinomas". Oncology Reports 25.2 (2011): 453-459.
Chicago
Kuwabara, T., Hiyama, T., Tanaka, S., Yoshihara, M., Arihiro, K., Chayama, K."Genetic pathways of multiple esophageal squamous cell carcinomas". Oncology Reports 25, no. 2 (2011): 453-459. https://doi.org/10.3892/or.2010.1110