Prognostic implications of microRNA-100 and its functional roles in human epithelial ovarian cancer

  • Authors:
    • Dong-Xian Peng
    • Min Luo
    • Li-Wen Qiu
    • Yuan-Li He
    • Xue-Feng Wang
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  • Published online on: January 11, 2012     https://doi.org/10.3892/or.2012.1625
  • Pages: 1238-1244
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Abstract

Dysregulation of microRNAs (miRNAs) has been found to be associated with a variety of diseases, including epithelial ovarian cancer (EOC). Recently, miR-100 was reported to be downregulated in human ovarian carcinoma, however, the clinical significance and functional roles of miR-100 expression in human EOC are unclear. TaqMan real-time quantitative RT-PCR assay was performed to detect the expression of miR-100 in 98 EOC tissues and 15 adjacent normal epithelial tissues. The relationship between miR-100 expression and clinicopathological factors in 98 EOC patients was statistically analyzed. The effect of miR-100 expression on patient survival was determined. Finally, the role of miR-100 in EOC cell growth and its possible mechanisms were analyzed with miR-100 precursor or inhibitor-transfected cells. We showed that the level of miR-100 was significantly lower in EOC tissues compared to adjacent normal tissues. Low miR-100 expression was found to be closely correlated with advanced FIGO stage, higher serum CA125 expression level and lymph node involvement. Also, low miR-100 expression was correlated with shorter overall survival of EOC patients, and multivariate analysis showed that the status of miR-100 expression was an independent predictor of overall survival in EOC. Additionally, miR-100 could affect the growth of EOC cells by post-transcriptionally regulating polo-like kinase 1 (PLK1) expression. Together, these results suggest that low miR-100 expression may be an independent poor prognostic factor and miR-100 can function as a tumor suppressor by targeting PLK1 in human EOCs.
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April 2012
Volume 27 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Peng D, Luo M, Qiu L, He Y and Wang X: Prognostic implications of microRNA-100 and its functional roles in human epithelial ovarian cancer. Oncol Rep 27: 1238-1244, 2012
APA
Peng, D., Luo, M., Qiu, L., He, Y., & Wang, X. (2012). Prognostic implications of microRNA-100 and its functional roles in human epithelial ovarian cancer. Oncology Reports, 27, 1238-1244. https://doi.org/10.3892/or.2012.1625
MLA
Peng, D., Luo, M., Qiu, L., He, Y., Wang, X."Prognostic implications of microRNA-100 and its functional roles in human epithelial ovarian cancer". Oncology Reports 27.4 (2012): 1238-1244.
Chicago
Peng, D., Luo, M., Qiu, L., He, Y., Wang, X."Prognostic implications of microRNA-100 and its functional roles in human epithelial ovarian cancer". Oncology Reports 27, no. 4 (2012): 1238-1244. https://doi.org/10.3892/or.2012.1625