microRNA-1301-mediated inhibition of tumorigenesis

Fang,Lin; Yang,Ning; Ma,Jie; Fu,Yong; Yang,Guang-Shun

doi:10.3892/or.2011.1589

microRNA-1301-mediated inhibition of tumorigenesis

Authors:
- Lin Fang
- Ning Yang
- Jie Ma
- Yong Fu
- Guang-Shun Yang
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Affiliations: Fifth Department of Liver Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, P.R. China, Fifth Department of Liver Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Chang Hai Road, Shanghai 200438, P.R. China
Published online on: December 12, 2011 https://doi.org/10.3892/or.2011.1589
Pages: 929-934
Copyright: © Fang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The relatively recent discovery of microRNAs has added a completely new dimension to the study of the regulation of tumor cells, but how they control cell behavior remains largely elusive. HepG2 cells were assigned to the miR-1301 group and the control group. RT-PCR, Western blotting, wound healing, the Transwell chamber migration and MTT assays, and apoptosis detection assays were used to analyze cell behavior of HepG2 cells after miR-1301 mimic transfection. Our study showed that miR-1301 was downregulated in HepG2 cells, and that miR-1301 inhibited migration and invasion of HepG2 cells and promoted cellular apoptosis after transfection with miR-1301 mimics. In addition, p53 mRNA and p53 protein expression was upregulated, and Bcl-2 and Bcl-xL mRNA and protein expression was downregulated in the miR-1301 group. These results indicate that miR-1301 may be an inhibitor of tumorigenesis in HepG2 cells.

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