Induction of apoptosis by D-limonene is mediated by inactivation of Akt in LS174T human colon cancer cells

  • Authors:
    • Shu-Sheng Jia
    • Guang-Peng Xi
    • Ming Zhang
    • Yan-Bo Chen
    • Bo Lei
    • Xin-Shu Dong
    • Yan-Mei Yang
  • View Affiliations

  • Published online on: October 19, 2012     https://doi.org/10.3892/or.2012.2093
  • Pages: 349-354
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Abstract

D-limonene is recognized as a potential chemotherapeutic agent, however, the details of this mechanism remain unclear. In this study, we investigated the effects of d-limonene on colon cancer cell viability and its potential mechanism of action in vitro. After 48 h of treatment, d-limonene suppressed the viability of LS174T cells in a dose-dependent manner and caused a dose-dependent apoptotic cell death. D-limonene activated caspase-3 and -9 and PARP cleavage in a dose-dependent manner. Moreover, an increase in Bax protein and cytosol cytochrome c from mitochondria and a decrease in bcl-2 protein were observed following treatment with d-limonene. In addition, d-limonene decreased the levels of p-Akt (Ser473), p-Akt (Thr308) and p-GSK-3β (Ser9), suggesting that d-limonene induced apoptosis via the mitochondrial death pathway and the suppression of the PI3K/Akt pathway.
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December 2012
Volume 29 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Jia, S., Xi, G., Zhang, M., Chen, Y., Lei, B., Dong, X., & Yang, Y. (2012). Induction of apoptosis by D-limonene is mediated by inactivation of Akt in LS174T human colon cancer cells. Oncology Reports, 29, 349-354. https://doi.org/10.3892/or.2012.2093
MLA
Jia, S., Xi, G., Zhang, M., Chen, Y., Lei, B., Dong, X., Yang, Y."Induction of apoptosis by D-limonene is mediated by inactivation of Akt in LS174T human colon cancer cells". Oncology Reports 29.1 (2012): 349-354.
Chicago
Jia, S., Xi, G., Zhang, M., Chen, Y., Lei, B., Dong, X., Yang, Y."Induction of apoptosis by D-limonene is mediated by inactivation of Akt in LS174T human colon cancer cells". Oncology Reports 29, no. 1 (2012): 349-354. https://doi.org/10.3892/or.2012.2093