Apoptotic markers in a prostate cancer cell line: Effect of ellagic acid

  • Authors:
    • Luca Vanella
    • Claudia Di Giacomo
    • Rosaria Acquaviva
    • Ignazio Barbagallo
    • Venera Cardile
    • Dong Hyun Kim
    • Nader G. Abraham
    • Valeria Sorrenti
  • View Affiliations

  • Published online on: September 30, 2013     https://doi.org/10.3892/or.2013.2757
  • Pages: 2804-2810
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Abstract

Ellagic acid (EA) inhibits cell growth and induces apoptosis in cultured cells; however, the precise molecular mechanism involved in EA-induced apoptosis in prostate cancer cells is unknown. The aim of the present study was to delineate possible apoptotic pathway(s) involved in the EA-mediated chemotherapeutic effects in the LNCaP human prostatic cancer cell line. EA produced anti-proliferative effects through inhibition of rapamycin (mTOR) activation and a reduction in intracellular levels of β-catenin. Moreover, we demonstrated that EA induced apoptosis via downregulation of the anti-apoptotic proteins, silent information regulator 1 (SIRT1), human antigen R (HuR) and heme oxygenase-1 (HO-1). EA modulated the expression of apoptosis-inducing factor (AIF) resulting in a significant increase in reactive oxygen species (ROS) levels and the activation of caspase-3. Finally, we demonstrated that EA reduced both transforming growth factor-β (TGF-β) and interleukin-6 (IL-6) levels. EA treatment resulted in the increased expression of the tumor suppressor protein p21 and increased the percentage of apoptotic cells. In conclusion, the results suggest that EA treatment represents a new and highly effective strategy in reducing prostate cancer carcinogenesis.
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December 2013
Volume 30 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Vanella L, Di Giacomo C, Acquaviva R, Barbagallo I, Cardile V, Kim DH, Abraham NG and Sorrenti V: Apoptotic markers in a prostate cancer cell line: Effect of ellagic acid. Oncol Rep 30: 2804-2810, 2013.
APA
Vanella, L., Di Giacomo, C., Acquaviva, R., Barbagallo, I., Cardile, V., Kim, D.H. ... Sorrenti, V. (2013). Apoptotic markers in a prostate cancer cell line: Effect of ellagic acid. Oncology Reports, 30, 2804-2810. https://doi.org/10.3892/or.2013.2757
MLA
Vanella, L., Di Giacomo, C., Acquaviva, R., Barbagallo, I., Cardile, V., Kim, D. H., Abraham, N. G., Sorrenti, V."Apoptotic markers in a prostate cancer cell line: Effect of ellagic acid". Oncology Reports 30.6 (2013): 2804-2810.
Chicago
Vanella, L., Di Giacomo, C., Acquaviva, R., Barbagallo, I., Cardile, V., Kim, D. H., Abraham, N. G., Sorrenti, V."Apoptotic markers in a prostate cancer cell line: Effect of ellagic acid". Oncology Reports 30, no. 6 (2013): 2804-2810. https://doi.org/10.3892/or.2013.2757