Clinical and biological impact of cyclin-dependent kinase subunit 2 in esophageal squamous cell carcinoma

Kita,Yoshiaki; Nishizono,Yuka; Okumura,Hiroshi; Uchikado,Yasuto; Sasaki,Ken; Matsumoto,Masataka; Setoyama,Tetsuro; Tanoue,Kiyonori; Omoto,Itaru; Mori,Shinichiro; Owaki,Tetsuhiro; Ishigami,Sumiya; Nakagawa,Hiroshi; Tanaka,Fumiaki; Mimori,Koshi; Mori,Masaki; Natsugoe,Shoji

doi:10.3892/or.2014.3062

Clinical and biological impact of cyclin-dependent kinase subunit 2 in esophageal squamous cell carcinoma

Authors:
- Yoshiaki Kita
- Yuka Nishizono
- Hiroshi Okumura
- Yasuto Uchikado
- Ken Sasaki
- Masataka Matsumoto
- Tetsuro Setoyama
- Kiyonori Tanoue
- Itaru Omoto
- Shinichiro Mori
- Tetsuhiro Owaki
- Sumiya Ishigami
- Hiroshi Nakagawa
- Fumiaki Tanaka
- Koshi Mimori
- Masaki Mori
- Shoji Natsugoe
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Affiliations: Department of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medicine, Kagoshima University, Kagoshima 890-8520, Japan, Division of Gastroenterology, Department of Medicine, Abramson Cancer Center, Perelman School of Medicine, Philadelphia, PA 19104, USA, Department of Surgery, Bepppu Hospital, Kyushu University, Beppu 874-0838, Japan, Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita 565-0871, Japan
Published online on: March 5, 2014 https://doi.org/10.3892/or.2014.3062
Pages: 1986-1992

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Abstract

Cyclin-dependent kinase subunit 2 (CKS2) is a cyclin-dependent kinase subunit (CKS) family member that participates in cell cycle regulation. Few studies have investigated its involvement in esophageal squamous cell carcinoma (ESCC). The aim of the present study was to assess the clinical significance of CKS2 in ESCC. We used immunohistochemistry to study the clinicopathologic significance of CKS2 protein expression in 121 patients with ESCC. Using real-time reverse transcriptase-polymerase chain reaction (RT-PCR), we examined the expression of CKS2 mRNA in tumors and the corresponding normal esophageal tissues that were obtained from 62 patients. Finally, siRNA-mediated attenuation of CKS2 expression was examined in vitro. CKS2 protein expression was significantly correlated with depth of tumor invasion, clinical stage, lymphatic invasion and distant metastasis (p=0.033, 0.028, 0.041 and 0.009, respectively). CKS2 mRNA expression was higher in cancer tissue than in corresponding normal tissue (p<0.001). Patients with positive-CKS2 protein expression had a poorer five year survival frequency than patients who did not express CKS2 protein (p=0.025). In vitro, siRNA-mediated suppression of CKS2 slowed the growth rate of ESCC cells compared to control cells (p<0.001). The evaluation of CKS2 expression is useful for predicting the cause of malignant tumors and the prognosis of patients with ESSC.

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