MicroRNA-127-3p promotes glioblastoma cell migration and invasion by targeting the tumor-suppressor gene SEPT7

Jiang,Huawei; Hua,Dasong; Zhang,Jing; Lan,Qing; Huang,Qiang; Yoon,Jae-Geun; Han,Xu; Li,Lisha; Foltz,Gregory; Zheng,Shu; Lin,Biaoyang

doi:10.3892/or.2014.3055

MicroRNA-127-3p promotes glioblastoma cell migration and invasion by targeting the tumor-suppressor gene SEPT7

Authors:
- Huawei Jiang
- Dasong Hua
- Jing Zhang
- Qing Lan
- Qiang Huang
- Jae-Geun Yoon
- Xu Han
- Lisha Li
- Gregory Foltz
- Shu Zheng
- Biaoyang Lin
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Affiliations: Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Afﬁliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China, Systems Biology Division, Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang 310029, P.R. China, Department of Neurosurgery and Brain Tumor Research Laboratory, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Swedish Neuroscience Institute, Swedish Medical Center, Seattle, WA 98122, USA
Published online on: March 5, 2014 https://doi.org/10.3892/or.2014.3055
Pages: 2261-2269

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Abstract

MicroRNAs (miRNAs) are small non-coding RNAs of 20-25 nucleotides in length that are capable of modulating gene expression post-transcriptionally. The potential roles of miRNAs in the tumorigenesis of glioblastoma (GBM) have been under intensive studies in the past few years. In the present study, we found a positive correlation between the levels of miR-127-3p and the cell migration and invasion abilities in several human GBM cell lines. We showed that miR-127-3p promoted cell migration and invasion of GBM cells using in vitro cell lines and in vivo mouse models. We identified SEPT7, a known tumor-suppressor gene that has been reported to suppress GBM cell migration and invasion, as a direct target of miR-127-3p. SEPT7 was able to partially abrogate the effect of miR-127-3p on cell migration and invasion. In addition, microarray analysis revealed that miR-127-3p regulated a number of migration and invasion-related genes. Finally, we verified that miR-127-3p affected the remodeling of the actin cytoskeleton mediated by SEPT7 in GBM cells.

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