Prognostic significance of miR-218 in human hepatocellular carcinoma and its role in cell growth

  • Authors:
    • Kangsheng Tu
    • Chao Li
    • Xin Zheng
    • Wei Yang
    • Yingmin Yao
    • Qingguang Liu
  • View Affiliations

  • Published online on: August 7, 2014     https://doi.org/10.3892/or.2014.3386
  • Pages: 1571-1577
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

MicroRNA-218 (miR-218) is considered a tumor suppressor in human cancer. In the present study, miR-218 expression was found to be significantly lower in human hepatocellular carcinoma (HCC) than in normal tumor-adjacent tissues. miR-218 was clearly silenced or downregulated in five HCC cells (HepG2, Hep3B, SMMC-7721, Huh7 and Bel-7402) compared with normal hepatocytes (LO2). The low expression of miR-218 conferred a poor 5-year survival in HCC patients. Multivariate Cox regression analysis indicated that miR-218 was an independent prognostic factor in HCC. Ectopic expression of miR-218 inhibited proliferation and promoted apoptosis in HepG2 and SMMC-7721 cells. In tumor bearing mice, miR-218 slowed down tumor growth by inducing apoptosis and growth arrest. Restoring miR-218 expression resulted in downregulation of B lymphoma Mo-MLV insertion region 1 homolog (BMI-1) mRNA and protein level in HepG2 and SMMC-7721 cells. In addition, BMI-1 mRNA expression in HCC was significantly higher than that in non-cancerous tissues. BMI-1 mRNA was inversely correlated with miR-218 expression in HCC tissues. In conclusion, miR-218 may serve as a prognostic biomarker and induce apoptosis and growth arrest by downregulating BMI-1 in HCC.
View Figures
View References

Related Articles

Journal Cover

October 2014
Volume 32 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Tu K, Li C, Zheng X, Yang W, Yao Y and Liu Q: Prognostic significance of miR-218 in human hepatocellular carcinoma and its role in cell growth. Oncol Rep 32: 1571-1577, 2014.
APA
Tu, K., Li, C., Zheng, X., Yang, W., Yao, Y., & Liu, Q. (2014). Prognostic significance of miR-218 in human hepatocellular carcinoma and its role in cell growth. Oncology Reports, 32, 1571-1577. https://doi.org/10.3892/or.2014.3386
MLA
Tu, K., Li, C., Zheng, X., Yang, W., Yao, Y., Liu, Q."Prognostic significance of miR-218 in human hepatocellular carcinoma and its role in cell growth". Oncology Reports 32.4 (2014): 1571-1577.
Chicago
Tu, K., Li, C., Zheng, X., Yang, W., Yao, Y., Liu, Q."Prognostic significance of miR-218 in human hepatocellular carcinoma and its role in cell growth". Oncology Reports 32, no. 4 (2014): 1571-1577. https://doi.org/10.3892/or.2014.3386