Alteration of CD4 T cell subsets in metastatic lymph nodes of human gastric cancer

  • Authors:
    • Yoshihiro Okita
    • Masaichi Ohira
    • Hiroaki Tanaka
    • Mao Tokumoto
    • Yukie Go
    • Katsunobu Sakurai
    • Takahiro Toyokawa
    • Naoshi Kubo
    • Kazuya Muguruma
    • Tetsuji Sawada
    • Kiyoshi Maeda
    • Kosei Hirakawa
  • View Affiliations

  • Published online on: June 15, 2015     https://doi.org/10.3892/or.2015.4064
  • Pages: 639-647
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Studies of tumor-infiltrating immune cells have revealed that immune escape plays an important role in tumor growth. The aim of the present study was to investigate the impact of metastasis affecting CD4+ T cell subsets in human clinical samples. Single-cell suspensions derived from tumor-draining lymph node (TDLN) and primary cancer specimens were assessed by flow cytometry, qRT-PCR and immunohistochemistry. In the CD4+ T cell subsets detected in TDLN, effector T cells (TE) in metastatic TDLN (mTDLN) was significantly lower than that in metastatic-free TDLN (mfTDLN). TE in mfTDLN were increased compared with normal controls. Similarly, effector memory T cells (TEM) in mTDLN was significantly lower than in control and mfTDLN. There was a significantly positive correlation between the proportion of TEM in TDLN and number of tumor-infiltrating CD4+ and CD8+ T cells. Th1 to Th2 ratio was lower in mTDLN, and Treg in mTDLN was significantly higher than in mfTDLN. CD4+ T cell and TE subsets in TDLN were significantly affected by metastasis. Immunosuppressive cells exhibit increased migration to TDLN, in which a subset of CD4+ TE is skewed towards immune tolerance in the tumor microenvironment.
View Figures
View References

Related Articles

Journal Cover

August-2015
Volume 34 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Okita Y, Ohira M, Tanaka H, Tokumoto M, Go Y, Sakurai K, Toyokawa T, Kubo N, Muguruma K, Sawada T, Sawada T, et al: Alteration of CD4 T cell subsets in metastatic lymph nodes of human gastric cancer. Oncol Rep 34: 639-647, 2015
APA
Okita, Y., Ohira, M., Tanaka, H., Tokumoto, M., Go, Y., Sakurai, K. ... Hirakawa, K. (2015). Alteration of CD4 T cell subsets in metastatic lymph nodes of human gastric cancer. Oncology Reports, 34, 639-647. https://doi.org/10.3892/or.2015.4064
MLA
Okita, Y., Ohira, M., Tanaka, H., Tokumoto, M., Go, Y., Sakurai, K., Toyokawa, T., Kubo, N., Muguruma, K., Sawada, T., Maeda, K., Hirakawa, K."Alteration of CD4 T cell subsets in metastatic lymph nodes of human gastric cancer". Oncology Reports 34.2 (2015): 639-647.
Chicago
Okita, Y., Ohira, M., Tanaka, H., Tokumoto, M., Go, Y., Sakurai, K., Toyokawa, T., Kubo, N., Muguruma, K., Sawada, T., Maeda, K., Hirakawa, K."Alteration of CD4 T cell subsets in metastatic lymph nodes of human gastric cancer". Oncology Reports 34, no. 2 (2015): 639-647. https://doi.org/10.3892/or.2015.4064