Cucurbitacin B reverses multidrug resistance by targeting CIP2A to reactivate protein phosphatase 2A in MCF-7/adriamycin cells

  • Authors:
    • Fen Cai
    • Liang Zhang
    • Xiangling Xiao
    • Chao Duan
    • Qiuyue Huang
    • Chunsheng Fan
    • Jian Li
    • Xuewen Liu
    • Shan Li
    • Ying Liu
  • View Affiliations

  • Published online on: June 22, 2016     https://doi.org/10.3892/or.2016.4892
  • Pages: 1180-1186
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Abstract

Cancerous inhibitor of protein phosphatase 2A (CIP2A) is a human oncoprotein that is overexpressed in various tumors. A previous study found that CIP2A expression is associated with doxorubicin (Dox) resistance. In the present study, we investigated whether cucurbitacin B (CuB), a natural anticancer compound found in Cucurbitaceae, reversed multidrug resistance (MDR) and downregulated CIP2A expression in MCF-7/Adriamycin (MCF-7/Adr) cells, a human breast multidrug-resistant cancer cell line. CuB treatment significantly suppressed MCF-7/Adr cell proliferation, and reversed Dox resistance. CuB treatment also induced caspase-dependent apoptosis, decreased phosphorylation of Akt (pAkt). The suppression of pAkt was mediated through CuB-induced activation of protein phosphatase 2A (PP2A). Furthermore, CuB activated PP2A through the suppression of CIP2A. Silencing CIP2A enhanced CuB-induced growth inhibition, apoptosis and MDR inhibition in MCF-7/Adr cells. In conclusion, we found that enhancement of PP2A activity by inhibition of CIP2A promotes the reversal of MDR induced by CuB.
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August-2016
Volume 36 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Cai F, Zhang L, Xiao X, Duan C, Huang Q, Fan C, Li J, Liu X, Li S, Liu Y, Liu Y, et al: Cucurbitacin B reverses multidrug resistance by targeting CIP2A to reactivate protein phosphatase 2A in MCF-7/adriamycin cells. Oncol Rep 36: 1180-1186, 2016
APA
Cai, F., Zhang, L., Xiao, X., Duan, C., Huang, Q., Fan, C. ... Liu, Y. (2016). Cucurbitacin B reverses multidrug resistance by targeting CIP2A to reactivate protein phosphatase 2A in MCF-7/adriamycin cells. Oncology Reports, 36, 1180-1186. https://doi.org/10.3892/or.2016.4892
MLA
Cai, F., Zhang, L., Xiao, X., Duan, C., Huang, Q., Fan, C., Li, J., Liu, X., Li, S., Liu, Y."Cucurbitacin B reverses multidrug resistance by targeting CIP2A to reactivate protein phosphatase 2A in MCF-7/adriamycin cells". Oncology Reports 36.2 (2016): 1180-1186.
Chicago
Cai, F., Zhang, L., Xiao, X., Duan, C., Huang, Q., Fan, C., Li, J., Liu, X., Li, S., Liu, Y."Cucurbitacin B reverses multidrug resistance by targeting CIP2A to reactivate protein phosphatase 2A in MCF-7/adriamycin cells". Oncology Reports 36, no. 2 (2016): 1180-1186. https://doi.org/10.3892/or.2016.4892