HBx-induced miR-21 suppresses cell apoptosis in hepatocellular carcinoma by targeting interleukin-12

  • Authors:
    • Dian Yin
    • Yilang Wang
    • Wenli Sai
    • Liang Zhang
    • Yajun Miao
    • Lili Cao
    • Xiaolu Zhai
    • Xiu Feng
    • Li Yang
  • View Affiliations

  • Published online on: August 17, 2016     https://doi.org/10.3892/or.2016.5026
  • Pages: 2305-2312
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Abstract

Hepatitis B virus (HBV) X protein (HBx) plays a key role in the initiation and progression of HBV infection‑induced hepatocellular carcinoma (HCC). Oncogenic microRNA-21 (miR-21) can be modulated by HBx protein in HCC. However, critical regulator genes in the pathway of HBx-induced miR-21 in HCC remain unclear. This study aimed to investigate the role of HBx-induced miR-21 in the apoptosis of HCC cells. In the study, interleukin-12 (IL-12) was demonstrated as a direct target of miR-21 by dual‑luciferase report assay, and miR-21 was highly expressed in HCC cells (HepG2 and HepG2 2.2.15) compared to L02 cells, but IL-12 was weakly expressed as detected by real-time quantitative PCR (RT-qPCR). Furthermore, miR-21 mimics, inhibitor, HBx-targeted siRNA, and the HBx overexpression vector (pHBx) were used to observe the regulatory effects of HBx-induced miR-21 via IL-12, and cell apoptosis was assessed. The results showed that overexpression of HBx resulted in the inhibition of IL-12. A high level of miR-21 resulted in a significant increase in proliferation and a decrease in IL-12 expression. Inhibition of miR-21 resulted in a significant increase in apoptosis and increased IL-12 expression. The results suggest that HCC cell apoptosis was suppressed at least partially through HBx-induced miR-21 by targeting IL-12.
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October-2016
Volume 36 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yin D, Wang Y, Sai W, Zhang L, Miao Y, Cao L, Zhai X, Feng X and Yang L: HBx-induced miR-21 suppresses cell apoptosis in hepatocellular carcinoma by targeting interleukin-12. Oncol Rep 36: 2305-2312, 2016
APA
Yin, D., Wang, Y., Sai, W., Zhang, L., Miao, Y., Cao, L. ... Yang, L. (2016). HBx-induced miR-21 suppresses cell apoptosis in hepatocellular carcinoma by targeting interleukin-12. Oncology Reports, 36, 2305-2312. https://doi.org/10.3892/or.2016.5026
MLA
Yin, D., Wang, Y., Sai, W., Zhang, L., Miao, Y., Cao, L., Zhai, X., Feng, X., Yang, L."HBx-induced miR-21 suppresses cell apoptosis in hepatocellular carcinoma by targeting interleukin-12". Oncology Reports 36.4 (2016): 2305-2312.
Chicago
Yin, D., Wang, Y., Sai, W., Zhang, L., Miao, Y., Cao, L., Zhai, X., Feng, X., Yang, L."HBx-induced miR-21 suppresses cell apoptosis in hepatocellular carcinoma by targeting interleukin-12". Oncology Reports 36, no. 4 (2016): 2305-2312. https://doi.org/10.3892/or.2016.5026