The transcription factor RFX5 is a transcriptional activator of the TPP1 gene in hepatocellular carcinoma

Zhao,Yangjing; Xie,Xingwang; Liao,Weijia; Zhang,Henghui; Cao,Hui; Fei,Ran; Wang,Xueyan; Wei,Lai; Shao,Qixiang; Chen,Hongsong

doi:10.3892/or.2016.5240

The transcription factor RFX5 is a transcriptional activator of the TPP1 gene in hepatocellular carcinoma

Authors:
- Yangjing Zhao
- Xingwang Xie
- Weijia Liao
- Henghui Zhang
- Hui Cao
- Ran Fei
- Xueyan Wang
- Lai Wei
- Qixiang Shao
- Hongsong Chen
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Affiliations: Department of Immunology, and The Key Laboratory of Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China, Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Disease, Beijing 100044, P.R. China, Laboratory of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China, Department of Pathology, The First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
Published online on: November 9, 2016 https://doi.org/10.3892/or.2016.5240
Pages: 289-296

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Abstract

Regulatory factor X-5 (RFX5) was previously characterized as an essential and highly specific regulator of major histocompatibility class II (MHCII) gene expression in the immune system. We found that RFX5 is significantly upregulated in hepatocellular carcinoma (HCC) tumors and cell lines compared with non-tumor tissues in mRNA expression levels, but it fails to induce the expression of MHCII. However, RFX5 can strongly bind to the tripeptidyl peptidase 1 (TPP1) promoter region and then increase its transcriptional activity. We also found that manipulation the expression of RFX5 can significantly affect the expression of TPP1 in HepG2, which suggested that RFX5 can transcriptionally activate TPP1 in HCC. Moreover, TPP1 is overexpressed in HCC tissues and significantly correlated with poor prognosis of HCC patients, suggesting that it may have potential biological implications in HCC.

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