Trichostatin A increases radiosensitization of tongue squamous cell carcinoma via miR‑375

  • Authors:
    • Lingfei Jia
    • Shan Zhang
    • Yiping Huang
    • Yunfei Zheng
    • Yehua Gan
  • View Affiliations

  • Published online on: November 18, 2016     https://doi.org/10.3892/or.2016.5261
  • Pages: 305-312
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Abstract

Trichostatin A (TSA), a histone deacetylase inhibitor, is used as an anti-carcinogenic and radiosensitizing agent in various cancers. However, the role and mechanism underlying its radiosensitization of tongue squamous cell carcinoma (TSCC) remains unclear. Thus, in this study we aimed to confirm the promotion of miR‑375 expression by TSA, and to investigate the effects of TSA and miR‑375 in the radiosensitivity of TSCC cells. The results showed that TSA had significant radiosensitizing effects on TSCC cells and miR‑375 overexpression had effects similar to TSA in sensitizing these cells to radiotherapy. By contrast, miR‑375 knockdown attenuated apoptosis induced by radiation combined with TSA. Mechanistically, the histone acetylation status of the miR‑375 promoter region was increased by TSA, resulting in the upregulation of miR‑375, which led to a decline of PDK1 and phosphorylated AKT. Taken together, our data suggest that TSA increases the radiosensitization and apoptosis in TSCC cells at least partially via miR‑375, and TSA or miR‑375 in combination with radiotherapy may provide a valuable approach for the treatment of TSCC.
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January-2017
Volume 37 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Jia L, Zhang S, Huang Y, Zheng Y and Gan Y: Trichostatin A increases radiosensitization of tongue squamous cell carcinoma via miR‑375. Oncol Rep 37: 305-312, 2017
APA
Jia, L., Zhang, S., Huang, Y., Zheng, Y., & Gan, Y. (2017). Trichostatin A increases radiosensitization of tongue squamous cell carcinoma via miR‑375. Oncology Reports, 37, 305-312. https://doi.org/10.3892/or.2016.5261
MLA
Jia, L., Zhang, S., Huang, Y., Zheng, Y., Gan, Y."Trichostatin A increases radiosensitization of tongue squamous cell carcinoma via miR‑375". Oncology Reports 37.1 (2017): 305-312.
Chicago
Jia, L., Zhang, S., Huang, Y., Zheng, Y., Gan, Y."Trichostatin A increases radiosensitization of tongue squamous cell carcinoma via miR‑375". Oncology Reports 37, no. 1 (2017): 305-312. https://doi.org/10.3892/or.2016.5261