Fibroblast growth factor 18 promotes proliferation and migration of H460 cells via the ERK and p38 signaling pathways

  • Authors:
    • Taotao Chen
    • Weiyue Gong
    • Haishan Tian
    • Haijun Wang
    • Shenghui Chu
    • Jisheng Ma
    • Huanhuan Yang
    • Jiliang Cheng
    • Min Liu
    • Xiaokun Li
    • Chao Jiang
  • View Affiliations

  • Published online on: December 7, 2016     https://doi.org/10.3892/or.2016.5301
  • Pages: 1235-1242
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Abstract

Recently, fibroblast growth factor 18 (FGF18) expression was reported to be upregulated in colon cancer and ovarian cancer, and increased expression of FGF18 mRNA and protein is associated with tumor progression and poor overall survival in patients; however, its role in lung cancer remains to be explored. In the present study, the effect and underlying molecular mechanisms of FGF18 on H460 cells were investigated. Cell proliferation and cell cycle alterations were detected using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay and flow cytometry. A wound healing assay was conducted to detect cell migration. Reverse transcription-quantitative polymerase chain reaction and western blotting were performed to measure extracellular signal-regulated kinase (ERK), p38 and matrix metalloproteinase 26 (MMP26) expression. Knockdown of FGF18 using short interfering RNA (siRNA-FGF18) suppressed H460 cell proliferation, inhibited cell migration via the downregulation of MMP26 levels, with siRNA-FGF18 additionally inhibiting the ERK and p38 signaling pathway. The present study indicates that FGF18 serves an essential role in the growth and migration of non-small cell lung cancer (NSCLC) cells by regulating the ERK, p38 signaling pathways and MMP26 protein levels, suggesting that FGF18 may be a potential molecular drug target for the treatment NSCLC.
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February-2017
Volume 37 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen T, Gong W, Tian H, Wang H, Chu S, Ma J, Yang H, Cheng J, Liu M, Li X, Li X, et al: Fibroblast growth factor 18 promotes proliferation and migration of H460 cells via the ERK and p38 signaling pathways. Oncol Rep 37: 1235-1242, 2017
APA
Chen, T., Gong, W., Tian, H., Wang, H., Chu, S., Ma, J. ... Jiang, C. (2017). Fibroblast growth factor 18 promotes proliferation and migration of H460 cells via the ERK and p38 signaling pathways. Oncology Reports, 37, 1235-1242. https://doi.org/10.3892/or.2016.5301
MLA
Chen, T., Gong, W., Tian, H., Wang, H., Chu, S., Ma, J., Yang, H., Cheng, J., Liu, M., Li, X., Jiang, C."Fibroblast growth factor 18 promotes proliferation and migration of H460 cells via the ERK and p38 signaling pathways". Oncology Reports 37.2 (2017): 1235-1242.
Chicago
Chen, T., Gong, W., Tian, H., Wang, H., Chu, S., Ma, J., Yang, H., Cheng, J., Liu, M., Li, X., Jiang, C."Fibroblast growth factor 18 promotes proliferation and migration of H460 cells via the ERK and p38 signaling pathways". Oncology Reports 37, no. 2 (2017): 1235-1242. https://doi.org/10.3892/or.2016.5301