Roles of immune inhibitory molecule B7-H4 in cervical cancer

  • Authors:
    • Sai Han
    • Yi Li
    • Jingjing Zhang
    • Lu Liu
    • Qian Chen
    • Qiuhong Qian
    • Shan Li
    • Youzhong Zhang
  • View Affiliations

  • Published online on: March 1, 2017     https://doi.org/10.3892/or.2017.5481
  • Pages: 2308-2316
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Abstract

Recent studies have reported that the immune-regulatory protein B7-H4 is highly expressed in various types of cancer, but little is known concerning its roles in cervical cancer. In the present study, we investigated the expression of B7-H4 in human tissues and serum samples, and explored the effects of B7-H4 on proliferation, apoptosis, migration and invasion of cervical cancer cell lines, including SiHa and HeLa. We found that B7-H4 was mainly located in the cytoplasm of cervical cancer cells as determined by immunofluorescence staining. Serum B7-H4 (sB7-H4) was overexpressed in patients with cervical intraepithelial neoplasia (CIN) and cervical cancer, and the area under the ROC curve (AUC) was 0.955. There was no statistical significance between B7-H4 expression and clinicopathological factors in cervical cancer tissue samples. B7-H4 promoted the proliferation of SiHa and HeLa cells, and protected them from apoptosis, which was related to the upregulation of E7, phosphorylated Rb (pRb), E2F, P16, P21, Bcl-2 and the downregulation of Rb, cleaved PARP and cleaved caspase-3 as determined by western blotting. In addition, B7-H4 increased the ability of cell migration and invasion by targeting angiogenic factors, matrix metalloproteinase (MMP)-2, MMP-9 and vascular endothelial growth factor (VEGF) as determined by RT-PCR. Our findings revealed that B7-H4 has the potential to be a useful prognostic marker. In addition, B7-H4 plays important roles in proliferation, apoptosis, migration and invasion, indicating that B7-H4 can serve as a new therapeutic target for cervical cancer.
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April-2017
Volume 37 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Han S, Li Y, Zhang J, Liu L, Chen Q, Qian Q, Li S and Zhang Y: Roles of immune inhibitory molecule B7-H4 in cervical cancer. Oncol Rep 37: 2308-2316, 2017
APA
Han, S., Li, Y., Zhang, J., Liu, L., Chen, Q., Qian, Q. ... Zhang, Y. (2017). Roles of immune inhibitory molecule B7-H4 in cervical cancer. Oncology Reports, 37, 2308-2316. https://doi.org/10.3892/or.2017.5481
MLA
Han, S., Li, Y., Zhang, J., Liu, L., Chen, Q., Qian, Q., Li, S., Zhang, Y."Roles of immune inhibitory molecule B7-H4 in cervical cancer". Oncology Reports 37.4 (2017): 2308-2316.
Chicago
Han, S., Li, Y., Zhang, J., Liu, L., Chen, Q., Qian, Q., Li, S., Zhang, Y."Roles of immune inhibitory molecule B7-H4 in cervical cancer". Oncology Reports 37, no. 4 (2017): 2308-2316. https://doi.org/10.3892/or.2017.5481