Open Access

Microarray expression profiles of long non-coding RNAs in germinal center-like diffuse large B-cell lymphoma

  • Authors:
    • Hong-Yu Gao
    • Bin Wu
    • Wei Yan
    • Zi-Mu Gong
    • Qi Sun
    • Hui-Han Wang
    • Wei Yang
  • View Affiliations

  • Published online on: July 14, 2017     https://doi.org/10.3892/or.2017.5821
  • Pages: 1363-1372
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non-coding RNAs (lncRNAs) are continuously transcribed and are involved in various cellular activities. However, their contributions to the occurrence and development of germinal center B-cell (GCB)-like diffuse large B-cell lymphoma (DLBCL) remain largely unknown. We applied microarray technology to profile the expression of lncRNAs in two different GCB-DLBCL cell lines (OCI-ly1 and OCI-ly19) and normal B lymphocytes. We demonstrated that 21,539 lncRNAs were expressed in all of the samples analyzed. This included 1,648 lncRNAs that showed a ≥2-fold upregulation and 2,671 lncRNAs that displayed a ≥2-fold downregulation in tumor cell lines (P<0.05). The expression levels of 8 lncRNAs were validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Bioinformatic analyses (Gene Ontology, pathway and network analysis) were performed to predict how the differentially expressed lncRNAs may function in GCB-DLBCL. Results from the pathway analysis suggested that totals of 64 and 62 biological pathways corresponded to upregulated and downregulated transcripts, respectively (P<0.05). Additionally, we constructed a lncRNA‑mRNA network for the purpose of identifying specific coding genes which were co-expressed with 5 selected lncRNAs. Conclusively, our results may contribute to a better understanding of GCB-DLBCL pathogenesis.
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September-2017
Volume 38 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Gao H, Wu B, Yan W, Gong Z, Sun Q, Wang H and Yang W: Microarray expression profiles of long non-coding RNAs in germinal center-like diffuse large B-cell lymphoma. Oncol Rep 38: 1363-1372, 2017
APA
Gao, H., Wu, B., Yan, W., Gong, Z., Sun, Q., Wang, H., & Yang, W. (2017). Microarray expression profiles of long non-coding RNAs in germinal center-like diffuse large B-cell lymphoma. Oncology Reports, 38, 1363-1372. https://doi.org/10.3892/or.2017.5821
MLA
Gao, H., Wu, B., Yan, W., Gong, Z., Sun, Q., Wang, H., Yang, W."Microarray expression profiles of long non-coding RNAs in germinal center-like diffuse large B-cell lymphoma". Oncology Reports 38.3 (2017): 1363-1372.
Chicago
Gao, H., Wu, B., Yan, W., Gong, Z., Sun, Q., Wang, H., Yang, W."Microarray expression profiles of long non-coding RNAs in germinal center-like diffuse large B-cell lymphoma". Oncology Reports 38, no. 3 (2017): 1363-1372. https://doi.org/10.3892/or.2017.5821