Scutellaria barbata D. Don inhibits 5-fluorouracil resistance in colorectal cancer by regulating PI3K/AKT pathway

  • Authors:
    • Jiumao Lin
    • Jianyu Feng
    • Hong Yang
    • Zhaokun Yan
    • Qiongyu Li
    • Lihui Wei
    • Zijun Lai
    • Yiyi Jin
    • Jun Peng
  • View Affiliations

  • Published online on: August 9, 2017     https://doi.org/10.3892/or.2017.5892
  • Pages: 2293-2300
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Abstract

5-Fluorouracil (5-FU) resistance or multidrug resistance (MDR) has become a major obstacle in clinical treatment of cancers including colorectal cancer (CRC). Aberrant activation of phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) pathway may lead to unlimited growth and chemoresistance in CRC cells, which thus could be a promising therapeutic target. As a long-term used traditional Chinese folk-medicine, Scutellaria barbata D. Don (SB) processes specific anticancer activity, but its activity against cancer chemoresistance is less known. Therefore, using a 5-FU-resistant CRC cell line HCT-8/5-FU, in this study we evaluated the therapeutic efficacy of the ethanol extracts of SB (EESB) against 5-FU resistance and explored the possible molecular mechanisms. We found that EESB significantly suppressed proliferation and promoted apoptosis in HCT-8/5-FU cells. Additionally, EESB displayed remarkable effect enhancing the retention of the ATP-binding cassette (ABC) transporter substrate, rhodamine‑123 (Rh‑123) in HCT-8/5-FU cells. Furthermore, EESB obviously downregulated the expression of cyclin D1, Bcl-2 and ABCG2, while upregulated p21 and Bax expression. Moreover, EESB showed a prominent suppressive effect on the activation of PI3K/AKT pathway. The findings suggested that Scutellaria barbata D. Don was able to inhibit chemoresistance in colorectal cancer by suppression of the PI3K/AKT pathway.
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October-2017
Volume 38 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lin J, Feng J, Yang H, Yan Z, Li Q, Wei L, Lai Z, Jin Y and Peng J: Scutellaria barbata D. Don inhibits 5-fluorouracil resistance in colorectal cancer by regulating PI3K/AKT pathway. Oncol Rep 38: 2293-2300, 2017.
APA
Lin, J., Feng, J., Yang, H., Yan, Z., Li, Q., Wei, L. ... Peng, J. (2017). Scutellaria barbata D. Don inhibits 5-fluorouracil resistance in colorectal cancer by regulating PI3K/AKT pathway. Oncology Reports, 38, 2293-2300. https://doi.org/10.3892/or.2017.5892
MLA
Lin, J., Feng, J., Yang, H., Yan, Z., Li, Q., Wei, L., Lai, Z., Jin, Y., Peng, J."Scutellaria barbata D. Don inhibits 5-fluorouracil resistance in colorectal cancer by regulating PI3K/AKT pathway". Oncology Reports 38.4 (2017): 2293-2300.
Chicago
Lin, J., Feng, J., Yang, H., Yan, Z., Li, Q., Wei, L., Lai, Z., Jin, Y., Peng, J."Scutellaria barbata D. Don inhibits 5-fluorouracil resistance in colorectal cancer by regulating PI3K/AKT pathway". Oncology Reports 38, no. 4 (2017): 2293-2300. https://doi.org/10.3892/or.2017.5892