Paclitaxel-mediated human aryl hydrocarbon receptor mRNA translation by an internal ribosomal entry site-dependent mechanism

  • Authors:
    • Wen-Qing Gao
    • Jing Ma
    • Liu-Liu Sun
    • Qi Li
    • Rui-Yu Zhu
    • Jian Jin
  • View Affiliations

  • Published online on: September 18, 2017     https://doi.org/10.3892/or.2017.5958
  • Pages: 3211-3219
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Abstract

The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that is best known in mediating the toxicities of dioxins and dioxin-like compounds. AHR is activated by a variety of endogenous ligands and participating in tumor development. Thus, it will provide a new approach for cancer prevention and treatment to study the translation mechanism of AHR in tumor cells. In this study, we show that the 5'-untranslated region (UTR) of AHR mRNA contains an internal ribosome entry site (IRES). After mapping the entire AHR 5'-UTR, we determined that the full-length 5'-UTR is indispensable for the highest IRES activity. Interestingly, we found that AHR expression is induced in ovarian (A2780), breast (MDA-MB231), hepatic (Bel7402) and colorectal cancer cells (SW620) by chemotherapeutic drug paclitaxel (PTX) through IRES-dependent translation mechanism. Moreover, IRES activity is increased in the PTX-resistant ovarian cancer cells in which AHR protein expression was also enhanced. These results strongly suggest an important role for AHR IRES-dependent translation mechanism in cancer cell response to paclitaxel treatment.
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November-2017
Volume 38 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Gao W, Ma J, Sun L, Li Q, Zhu R and Jin J: Paclitaxel-mediated human aryl hydrocarbon receptor mRNA translation by an internal ribosomal entry site-dependent mechanism. Oncol Rep 38: 3211-3219, 2017.
APA
Gao, W., Ma, J., Sun, L., Li, Q., Zhu, R., & Jin, J. (2017). Paclitaxel-mediated human aryl hydrocarbon receptor mRNA translation by an internal ribosomal entry site-dependent mechanism. Oncology Reports, 38, 3211-3219. https://doi.org/10.3892/or.2017.5958
MLA
Gao, W., Ma, J., Sun, L., Li, Q., Zhu, R., Jin, J."Paclitaxel-mediated human aryl hydrocarbon receptor mRNA translation by an internal ribosomal entry site-dependent mechanism". Oncology Reports 38.5 (2017): 3211-3219.
Chicago
Gao, W., Ma, J., Sun, L., Li, Q., Zhu, R., Jin, J."Paclitaxel-mediated human aryl hydrocarbon receptor mRNA translation by an internal ribosomal entry site-dependent mechanism". Oncology Reports 38, no. 5 (2017): 3211-3219. https://doi.org/10.3892/or.2017.5958