LARP1 is regulated by the XIST/miR-374a axis and functions as an oncogene in non-small cell lung carcinoma

  • Authors:
    • Zhizheng Xu
    • Jing Xu
    • Huoquan Lu
    • Bin Lin
    • Shaopeng Cai
    • Jun Guo
    • Farong Zang
    • Rui Chen
  • View Affiliations

  • Published online on: October 17, 2017     https://doi.org/10.3892/or.2017.6040
  • Pages:3659-3667
Metrics: PDF 0 views | HTML 0 views
0

Abstract

La-related protein 1 (LARP1) is a conserved RNA-binding protein and is known to regulate 5'-terminal oligopyrimidine tract (TOP) mRNA translation. Dysregulated LARP1 has been reported to be related to the development of several cancers. However, the exact function and mechanism of LARP1 in non-small cell lung cancer (NSCLC) is largely unknown. In the present study, we found that the mRNA levels of LARP1 were increased in NSCLC cells compared to those in normal control cells. Knockdown of LARP1 inhibited cell proliferation, migration and invasion in NSCLC cells and tumourigenicity in H520 cells. Both in vitro and in vivo analyses confirmed that STAT3 signalling was inactivated by the knockdown of LARP1. Moreover, LARP1 was identified as a direct target of miR-374a. Overexpression of miR-374a attenuated the promotor effects of LARP1 by inhibiting proliferation, metastasis and STAT3 signalling. Clinically, LARP1 was markedly overexpressed in NSCLC tissues, and upregulated LARP1 was correlated with tumour progression and poor survival. The expression of miR-374a was negatively correlated with the expression of LARP1 in NSCLC tissues. Furthermore, we found that XIST functioned as a competing endogenous RNA to suppress miR-374a, which regulated its downstream target LARP1. In summary, we suggest that the dysfunction of the XIST/miR-374a/LARP1 axis contributes to NSCLC and may serve as a promising therapeutic strategy for treatment.

Related Articles

Journal Cover

December 2017
Volume 38 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

2016 Impact Factor: 2.662
Ranked #31/217 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Xu, Z., Xu, J., Lu, H., Lin, B., Cai, S., Guo, J. ... Chen, R. (2017). LARP1 is regulated by the XIST/miR-374a axis and functions as an oncogene in non-small cell lung carcinoma. Oncology Reports, 38, 3659-3667. https://doi.org/10.3892/or.2017.6040
MLA
Xu, Z., Xu, J., Lu, H., Lin, B., Cai, S., Guo, J., Zang, F., Chen, R."LARP1 is regulated by the XIST/miR-374a axis and functions as an oncogene in non-small cell lung carcinoma". Oncology Reports 38.6 (2017): 3659-3667.
Chicago
Xu, Z., Xu, J., Lu, H., Lin, B., Cai, S., Guo, J., Zang, F., Chen, R."LARP1 is regulated by the XIST/miR-374a axis and functions as an oncogene in non-small cell lung carcinoma". Oncology Reports 38, no. 6 (2017): 3659-3667. https://doi.org/10.3892/or.2017.6040