miR-16 mimics inhibit TGF-β1-induced epithelial-to-mesenchymal transition via activation of autophagy in non-small cell lung carcinoma cells

  • Authors:
    • Hui Wang
    • Ying Zhang
    • Qian Wu
    • Yong-Bin Wang
    • Wei Wang
  • View Affiliations

  • Published online on: November 9, 2017     https://doi.org/10.3892/or.2017.6088
  • Pages:247-254
Metrics: HTML 0 views | PDF 0 views
0

Abstract

Autophagy is critical for the metastasis of cancer cells through induction of epithelial-to-mesenchymal transition (EMT). Activation of TGF-β signaling plays a key role in regulating autophagy. miR-16 may be associated with non-small cell lung carcinoma (NSCLC) progression. However, the role of miR-16 in NSCLC cell autophagy in the presence of TGF-β and the underlying mechanism are still unclear. To test whether miR-16 targets ATG3 which is involved in autophagy of NSCLC cells, we studied the expression levels of miR-16 and ATG3 in NSCLC patients, verified the targeting of ATG3 by miR-16 by luciferase reporter gene system, and investigated the role of miR-16 in the autophagy of NSCLC cells. Results revealed that miR-16 was significantly downregulated, and ATG3 was significantly upregulated in NSCLC patient tissue samples. ATG3 was found to be a direct target of miR-16. TGF-β1 significantly downregulated the expression of miR-16 and ATG3 mRNA. Using transmission electron microscopy, we observed that TGF-β1 treatment reduced autophagosomes in the A549 cells, and miR-16 mimics increased the autophagosomes in the presence of TGF-β1. Acridine orange (AO) staining and expression of LC3B II/I and p62 confirmed the inhibition of autophagy by TGF-β1, and the recovery of TGF-β1-mediated inhibition of autophagy by miR-16 mimics. Finally, miR-16 mimics inhibited TGF-β1-induced EMT, and this effect was attenuated by autophagy inhibitor 3-MA. Taken together, miR-16 mimics inhibited TGF-β1-induced EMT via activation of autophagy.

Related Articles

Journal Cover

January 2018
Volume 39 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

2016 Impact Factor: 2.662
Ranked #31/217 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Wang, H., Zhang, Y., Wu, Q., Wang, Y., & Wang, W. (2018). miR-16 mimics inhibit TGF-β1-induced epithelial-to-mesenchymal transition via activation of autophagy in non-small cell lung carcinoma cells. Oncology Reports, 39, 247-254. https://doi.org/10.3892/or.2017.6088
MLA
Wang, H., Zhang, Y., Wu, Q., Wang, Y., Wang, W."miR-16 mimics inhibit TGF-β1-induced epithelial-to-mesenchymal transition via activation of autophagy in non-small cell lung carcinoma cells". Oncology Reports 39.1 (2018): 247-254.
Chicago
Wang, H., Zhang, Y., Wu, Q., Wang, Y., Wang, W."miR-16 mimics inhibit TGF-β1-induced epithelial-to-mesenchymal transition via activation of autophagy in non-small cell lung carcinoma cells". Oncology Reports 39, no. 1 (2018): 247-254. https://doi.org/10.3892/or.2017.6088