Downregulation of microRNA-95-3p suppresses cell growth of osteosarcoma via CDKN1A/p21 expression

  • Authors:
    • Xin Zhao
    • Yukai Yang
    • Jianting Xu
    • Yang Luo
    • Ying Xin
    • Yanbing Wang
  • View Affiliations

  • Published online on: November 1, 2017     https://doi.org/10.3892/or.2017.6065
  • Pages:289-297
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Abstract

The purpose of the present study was to examine the function of microRNA-95-3p on cell growth of osteosarcoma and to investigate its mechanism. Compared to healthy controls, the serum expression of microRNA-95-3p was effectively upregulated in patients with osteosarcoma. MicroRNA-95-3p expression in patients with osteosarcoma had significant association with clinical stage. Downregulation of microRNA-95-3p expression suppressed cell growth, induced apoptosis, increased caspase-3 and caspase-9 activities and Bax/Bcl-2 protein expression in osteosarcoma cells. The anticancer effects of microRNA-95-3p on cell growth of osteosarcoma cell suppressed TGF-β and p-Smad2 protein expression, induced p21 protein expression and suppressed cyclin D1 protein expression in osteosarcoma cells. Whereas, overexpression of microRNA-95-3p increased cell growth, and inhibited apoptosis of osteosarcoma cells through TGF-β/CDKN1A/p21/cyclin D1 expression. After promotion of TGF-β, the anticancer effects of microRNA-95-3p were effectively reversed in osteosarcoma cells. Taken together, our results demonstrate that downregulation of microRNA-95-3p suppresses cell growth of osteosarcoma via CDKN1A/p21 expression.

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January 2018
Volume 39 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

2016 Impact Factor: 2.662
Ranked #31/217 Oncology
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APA
Zhao, X., Yang, Y., Xu, J., Luo, Y., Xin, Y., & Wang, Y. (2018). Downregulation of microRNA-95-3p suppresses cell growth of osteosarcoma via CDKN1A/p21 expression. Oncology Reports, 39, 289-297. https://doi.org/10.3892/or.2017.6065
MLA
Zhao, X., Yang, Y., Xu, J., Luo, Y., Xin, Y., Wang, Y."Downregulation of microRNA-95-3p suppresses cell growth of osteosarcoma via CDKN1A/p21 expression". Oncology Reports 39.1 (2018): 289-297.
Chicago
Zhao, X., Yang, Y., Xu, J., Luo, Y., Xin, Y., Wang, Y."Downregulation of microRNA-95-3p suppresses cell growth of osteosarcoma via CDKN1A/p21 expression". Oncology Reports 39, no. 1 (2018): 289-297. https://doi.org/10.3892/or.2017.6065