Open Access

MicroRNA-505 is downregulated in human osteosarcoma and regulates cell proliferation, migration and invasion

Retraction in: /10.3892/or.2023.8582

  • Authors:
    • Yu-Jiang Liu
    • Wei Li
    • Feng Chang
    • Jian-Na Liu
    • Jun-Xin Lin
    • De-Xi Chen
  • View Affiliations

  • Published online on: December 11, 2017     https://doi.org/10.3892/or.2017.6142
  • Pages: 491-500
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Recent studies have demonstrated that microRNAs (miRNAs/miRs) are involved in osteosarcoma tumorigenesis, progression, invasion and metastasis. For example, miR-505 plays important roles in human carcinogenesis; however, its exact function in osteosarcoma remains unclear. MicroRNA profiles of osteosarcoma and normal tissues were obtained by miRNA microarray assays, which were validated by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Then, high-mobility group box 1 (HMGB1) expression was evaluated by qRT-PCR and western blot analysis. The correlation between miR-505 and HMGB1 was analyzed by Pearson correlation. In vitro, the biological functions of miR-505 were examined by wound healing, MTT and Transwell assays and western blot analysis in MG63 cells transfected with miRNA mimics or empty vector. Luciferase assay was utilized to assess whether HMGB1 is a target of miR-505. miRNA microarrays revealed 26 aberrant miRNAs in osteosarcoma tissues; miR-505 showed the most pronounced decrease (P<0.01), which was significantly associated with TNM stage and metastasis status (P<0.05). In addition, HMGB1 was highly expressed in osteosarcoma tissues (P<0.01), with a significantly negative correlation with miR-505 (r=-0.6679, P<0.001). Furthermore, miR-505 inhibited proliferation, migration and invasion abilities of MG63 cells (P<0.01). Moreover, luciferase activity of the HMGB1-3'-UTR plasmid was suppressed following miR-505 binding (P<0.01). Finally, HMGB1 overexpression partly reversed the effects of miR-505 on MG63 cells. In conclusion, miR-505 levels are decreased in osteosarcoma tissues, and reduced miR-505 expression is significantly associated with poorer clinical prognosis in patients with osteosarcomas. miR-505 inhibits osteosarcoma cell proliferation, migration and invasion by regulating HMGB1.
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February-2018
Volume 39 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Liu Y, Li W, Chang F, Liu J, Lin J and Chen D: MicroRNA-505 is downregulated in human osteosarcoma and regulates cell proliferation, migration and invasion Retraction in /10.3892/or.2023.8582. Oncol Rep 39: 491-500, 2018
APA
Liu, Y., Li, W., Chang, F., Liu, J., Lin, J., & Chen, D. (2018). MicroRNA-505 is downregulated in human osteosarcoma and regulates cell proliferation, migration and invasion Retraction in /10.3892/or.2023.8582. Oncology Reports, 39, 491-500. https://doi.org/10.3892/or.2017.6142
MLA
Liu, Y., Li, W., Chang, F., Liu, J., Lin, J., Chen, D."MicroRNA-505 is downregulated in human osteosarcoma and regulates cell proliferation, migration and invasion Retraction in /10.3892/or.2023.8582". Oncology Reports 39.2 (2018): 491-500.
Chicago
Liu, Y., Li, W., Chang, F., Liu, J., Lin, J., Chen, D."MicroRNA-505 is downregulated in human osteosarcoma and regulates cell proliferation, migration and invasion Retraction in /10.3892/or.2023.8582". Oncology Reports 39, no. 2 (2018): 491-500. https://doi.org/10.3892/or.2017.6142