Kynurenine promotes the goblet cell differentiation of HT-29 colon carcinoma cells by modulating Wnt, Notch and AhR signals

  • Authors:
    • Joo-Hung Park
    • Jeong-Min Lee
    • Eun-Jin Lee
    • Da-Jeong Kim
    • Won-Bhin Hwang
  • View Affiliations

  • Published online on: February 13, 2018     https://doi.org/10.3892/or.2018.6266
  • Pages: 1930-1938
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Abstract

Various amino acids regulate cell growth and differentiation. In the present study, we examined the ability of HT-29 cells to differentiate into goblet cells in RPMI and DMEM which are largely different in the amounts of numerous amino acids. Most of the HT-29 cells differentiated into goblet cells downregulating the stem cell marker Lgr5 when cultured in DMEM, but remained undifferentiated in RPMI. The goblet cell differentiation in DMEM was inhibited by 1-methyl-tryptophan (1-MT), an inhibitor of indoleamine 2,3 dioxygenase-1 which is the initial enzyme in tryptophan metabolism along the kynurenine (KN) pathway, whereas tryptophan and KN induced goblet cell differentiation in RPMI. The levels of Notch1 and its activation product Notch intracytoplasmic domain in HT-29 cells were lower in DMEM than those in RPMI and were increased by 1-MT in both media. HT-29 cells grown in both media expressed β-catenin at the same level on day 2 when goblet cell differentiation was not observed. β-catenin expression, which was increased by 1-MT in both media, was decreased by KN. DMEM reduced Hes1 expression while enhancing Hath1 expression. Finally, aryl hydrocarbon receptor (AhR) activation moderately induced goblet cell differentiation. Our results suggest that KN promotes goblet cell differentiation by regulating Wnt, Notch, and AhR signals and expression of Hes1 and Hath1.
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April-2018
Volume 39 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Park J, Lee J, Lee E, Kim D and Hwang W: Kynurenine promotes the goblet cell differentiation of HT-29 colon carcinoma cells by modulating Wnt, Notch and AhR signals. Oncol Rep 39: 1930-1938, 2018
APA
Park, J., Lee, J., Lee, E., Kim, D., & Hwang, W. (2018). Kynurenine promotes the goblet cell differentiation of HT-29 colon carcinoma cells by modulating Wnt, Notch and AhR signals. Oncology Reports, 39, 1930-1938. https://doi.org/10.3892/or.2018.6266
MLA
Park, J., Lee, J., Lee, E., Kim, D., Hwang, W."Kynurenine promotes the goblet cell differentiation of HT-29 colon carcinoma cells by modulating Wnt, Notch and AhR signals". Oncology Reports 39.4 (2018): 1930-1938.
Chicago
Park, J., Lee, J., Lee, E., Kim, D., Hwang, W."Kynurenine promotes the goblet cell differentiation of HT-29 colon carcinoma cells by modulating Wnt, Notch and AhR signals". Oncology Reports 39, no. 4 (2018): 1930-1938. https://doi.org/10.3892/or.2018.6266